The relationship between glial distortion and neuronal changes following intestinal ischemia and reperfusion

Thacker, M., Rivera, L.R., Cho, H.-J. and Furness, J.B. 2011, The relationship between glial distortion and neuronal changes following intestinal ischemia and reperfusion, Neurogastroenterology & motility, vol. 23, no. 11, pp. e500-e509, doi: 10.1111/j.1365-2982.2011.01696.x.

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Title The relationship between glial distortion and neuronal changes following intestinal ischemia and reperfusion
Author(s) Thacker, M.
Rivera, L.R.
Cho, H.-J.
Furness, J.B.
Journal name Neurogastroenterology & motility
Volume number 23
Issue number 11
Start page e500
End page e509
Total pages 10
Publisher Wiley-Blackwell
Place of publication Chichester, Eng.
Publication date 2011-11
ISSN 1350-1925
Keyword(s) enteric glia
enteric nervous system
glial fibrillary acidic protein
Summary Background Damage to mucosal epithelial cells, musclecells and enteric neurons has been extensivelystudied following intestinal ischemia and reperfusion(I/R). Interestingly, the effects of intestinal I/R on entericglia remains unexplored, despite knowledge thatglia contribute to neuronal maintenance. Here, we describestructural damage to enteric glia and associatedchanges in distribution and immunoreactivity of theneuronal protein Hu. Methods The mouse small intestinewas made ischemic for 3 h and reperfused from 1 to12 h. Immunohistochemical localisation of glial fibrillaryacidic protein (GFAP),HuandTUNELwere used toevaluate changes. Key Results At all time points glialcells became distorted, which was evident by theiraltered GFAP immunoreactivity, including an unusualappearance of bright perinuclear GFAP staining and thepresence of GFAP globules. The numbers of neurons perganglion area were significantly fewer in ganglia thatcontained distorted glia when compared with gangliathat contained glia of normal appearance. The distributionof Hu immunoreactivity was altered at all reperfusiontime points. The presence of vacuoles and Hugranules in neurons was evident and an increase innuclear Hu, relative to cytoplasmic Hu, was observed inganglia that contained both normal and distorted glialcells. A number of neurons appeared to lose their Huimmunoreactivity, most noticeably in ganglia thatcontained distorted glial cells. TUNEL reactionoccurred in a minority of glial cells and neurons.Conclusions & Inferences Structural damage to gliofilamentsoccurs following I/R and may be associatedwith damage to neighboring neurons.
Language eng
DOI 10.1111/j.1365-2982.2011.01696.x
Field of Research 110399 Clinical Sciences not elsewhere classified
1103 Clinical Sciences
1109 Neurosciences
Socio Economic Objective 920105 Digestive System Disorders
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2011, Blackwell Publishing
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Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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