Damaging effects of ischemia/reperfusion on intestinal muscle

Pontell, Louise, Sharma, Purnima, Rivera, Leni R., Thacker, Michelle, Tan, Yan Hong, Brock, James A. and Furness, John B. 2011, Damaging effects of ischemia/reperfusion on intestinal muscle, Cell and tissue research, vol. 343, no. 2, pp. 411-419, doi: 10.1007/s00441-010-1096-z.

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Title Damaging effects of ischemia/reperfusion on intestinal muscle
Author(s) Pontell, Louise
Sharma, Purnima
Rivera, Leni R.
Thacker, Michelle
Tan, Yan Hong
Brock, James A.
Furness, John B.
Journal name Cell and tissue research
Volume number 343
Issue number 2
Start page 411
End page 419
Total pages 9
Publisher Springer
Place of publication Heidelberg, Germany
Publication date 2011-02
ISSN 0302-766X
Keyword(s) ischemia
muscle degeneration
muscle repair
enteric neurons
mouse (male)
Summary Periods of ischemia followed by restoration of blood flow cause ischemia/reperfusion (I/R) injury. In the intestine, I/R damage to the mucosa and neurons is prominent. Functionally, abnormalities occur in motility, most conspicuously a slowing of transit, possibly as a consequence of damage to neurons and/or muscle. Here, we describe degenerative and regenerative changes that have not been previously reported in intestinal muscle. The mouse small intestine was made ischemic for 1 h, followed by re-perfusion for 1 h to 7 days. The tissues were examined histologically, after hematoxylin/eosin and Masson’s trichrome staining, and by myeloperoxidase histochemistry to detect inflammatory reactions to I/R. Histological analysis revealed changes in the mucosa, muscle, and neurons. The mucosa was severely but transiently damaged. The mucosal surface was sloughed off at 1–3 h, but re-epithelialization occurred by 12 h, and the epithelium appeared healthy by 1–2 days. Longitudinal muscle degeneration was followed by regeneration, but little effect on the circular muscle was noted. The first signs of muscle change were apparent at 3–12 h, and by 1 and 2 days, extensive degeneration within the muscle was observed, which included clear cytoplasm, pyknotic nuclei, and apoptotic bodies. The muscle recovered quickly and appeared normal at 7 days. Histological evidence of neuronal damage was apparent at 1–7 days. Neutrophils were not present in the muscle layers and were infrequent in the mucosa. However, they were often seen in the longitudinal muscle at 1–3 days and were also present in the circular muscle. Neutrophil numbers increased in the mucosa in both I/R and sham-operated animals and remained elevated from 1 h to 7 days. We conclude that I/R causes severe longitudinal muscle damage, which might contribute to the long-term motility deficits observed after I/R injury to the intestine.
Language eng
DOI 10.1007/s00441-010-1096-z
Field of Research 110399 Clinical Sciences not elsewhere classified
1116 Medical Physiology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2010, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30079273

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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