GM3 ganglioside and phosphatidylethanolamine-containing lipids are adipose tissue markers of insulin resistance in obese women

Wentworth, John M., Naselli, Gaetano, Ngui, Katrina, Smyth, Gordon K., Liu, Ruijie, O'Brien, Paul E., Bruce, Clinton, Weir, Jacqui, Cinel, Michelle, Meikle, Peter J. and Harrison, Leonard C. 2016, GM3 ganglioside and phosphatidylethanolamine-containing lipids are adipose tissue markers of insulin resistance in obese women, International journal of obesity, vol. 40, pp. 706-713, doi: 10.1038/ijo.2015.223.

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Title GM3 ganglioside and phosphatidylethanolamine-containing lipids are adipose tissue markers of insulin resistance in obese women
Author(s) Wentworth, John M.
Naselli, Gaetano
Ngui, Katrina
Smyth, Gordon K.
Liu, Ruijie
O'Brien, Paul E.
Bruce, ClintonORCID iD for Bruce, Clinton
Weir, Jacqui
Cinel, Michelle
Meikle, Peter J.
Harrison, Leonard C.
Journal name International journal of obesity
Volume number 40
Start page 706
End page 713
Total pages 8
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2016
ISSN 1476-5497
Keyword(s) Obesity
insulin resistance
lipid profiling
adipose tissue
Summary AimsThe association between central obesity and insulin resistance reflects the properties of visceral adipose tissue. Our aim was to gain further insight into this association by analysing the lipid composition of subcutaneous and omental adipose tissue in obese women with and without insulin resistance.MethodsSubcutaneous and omental adipose tissue and serum were obtained from 29 obese non-diabetic women, 13 of whom were hyperinsulinemic. Histology, and lipid and gene profiling were performed.ResultsIn omental adipose tissue of obese, insulin-resistant women, adipocyte hypertrophy and macrophage infiltration were accompanied by an increase in GM3 ganglioside and its synthesis enzyme ST3GAL5; in addition, phosphatidylethanolamine (PE) lipids were increased and their degradation enzyme, PEMT, decreased. ST3GAL5 was expressed predominantly in adipose stromovascular cells and PEMT in adipocytes. Insulin resistance was also associated with an increase in PE lipids in serum.InterpretationThe relevance of these findings to insulin resistance in humans is supported by published mouse studies in which adipocyte GM3 ganglioside, increased by the inflammatory cytokine tumour necrosis factor-α, impaired insulin action, and PEMT was required for adipocyte lipid storage. Thus, in visceral adipose tissue of obese humans, an increase in GM3 ganglioside secondary to inflammation may contribute to insulin resistance and a decrease in PEMT may be a compensatory response to adipocyte hypertrophy.International Journal of Obesity accepted article preview online, 26 October 2015. doi:10.1038/ijo.2015.223.
Language eng
DOI 10.1038/ijo.2015.223
Field of Research 110306 Endocrinology
Socio Economic Objective 920106 Endocrine Organs and Diseases (excl. Diabetes)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Nature Publishing Group
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Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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