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3T3-L1 adipocytes display phenotypic characteristics of multiple adipocyte lineages

Morrison, Shona and McGee, Sean L. 2015, 3T3-L1 adipocytes display phenotypic characteristics of multiple adipocyte lineages, Adipocyte, vol. 4, no. 4, pp. 295-302, doi: 10.1080/21623945.2015.1040612.

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Title 3T3-L1 adipocytes display phenotypic characteristics of multiple adipocyte lineages
Author(s) Morrison, Shona
McGee, Sean L.
Journal name Adipocyte
Volume number 4
Issue number 4
Start page 295
End page 302
Total pages 8
Publisher Taylor & Francis
Place of publication London, Eng.
Publication date 2015
ISSN 2162-3945
Keyword(s) 3T3-L1 adipocyte
UCP-1
beige adipocyte
norepinephrine
uncoupling
Summary Differentiated 3T3-L1 adipocytes are a widely used in vitro model of white adipocytes. In addition to classical white and brown adipocytes that are derived from different cell lineages, beige adipocytes have also been identified, which have characteristics of both white and brown adipocytes. Here we show that 3T3-L1 adipocytes display features of multiple adipocytes lineages. While the gene expression profile and basal bioenergetics of 3T3-L1 adipocytes was typical of white adipocytes, they responded acutely to catecholamines by increasing oxygen consumption in an UCP1-dependent manner, and by increasing the expression of genes enriched in brown but not beige adipocytes. Chronic exposure to catecholamines exacerbated this phenotype. However, a beige adipocyte differentiation procedure did not induce a beige adipocyte phenotype in 3T3-L1 fibroblasts. These multiple lineage features should be considered when interpreting data from experiments utilizing 3T3-L1 adipocytes.
Language eng
DOI 10.1080/21623945.2015.1040612
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2015, Taylor & Francis
Persistent URL http://hdl.handle.net/10536/DRO/DU:30079511

Document type: Journal Article
Collection: School of Medicine
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