A Pb<sup>2+</sup>-ion electrochemical biosensor based on single-stranded DNAzyme catalytic beacon

Zhang, Yanli, Xiao, Shixiu, Li, Haizhen, Liu, Hongjun, Pang, Pengfei, Wang, Hongbin, Wu, Zhan and Yang, Wenrong 2016, A Pb2+-ion electrochemical biosensor based on single-stranded DNAzyme catalytic beacon, Sensors and actuators B: chemical, vol. 222, pp. 1083-1089, doi: 10.1016/j.snb.2015.08.046.

Attached Files
Name Description MIMEType Size Downloads

Title A Pb2+-ion electrochemical biosensor based on single-stranded DNAzyme catalytic beacon
Author(s) Zhang, Yanli
Xiao, Shixiu
Li, Haizhen
Liu, Hongjun
Pang, Pengfei
Wang, Hongbin
Wu, Zhan
Yang, WenrongORCID iD for Yang, Wenrong orcid.org/0000-0001-8815-1951
Journal name Sensors and actuators B: chemical
Volume number 222
Start page 1083
End page 1089
Total pages 7
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-01
ISSN 0925-4005
Keyword(s) lead ion
single-stranded DNAzyme
electrochemical biosensor
Summary A novel strategy for selective and sensitive electrochemical lead ion (Pb2+) biosensor was developed based on the single-stranded DNAzyme catalytic beacon. A DNAzyme that requires Pb2+ for activation was selected and labeled with redox-active ferrocene (Fc) for signal transducer. The Fc-labeled single-stranded DNAzyme (Fc-ssDNAzyme) was self-assembled through SAu bonding on a gold electrode surface. In the presence of Pb2+, the ssDNAzyme was activated and catalyzed the hydrolytic cleavage of the substrate strand, resulting in the removal of the substrate strand along with the Fc from the Au electrode surface. The dissociation of Fc caused a decrease of electrochemical signal ("signal-off"). Under the optimal conditions, the electrochemical signal of Fc decreased directly with the increasing Pb2+ concentration, exhibiting a linear response in the range of 0.5nM to 5μM with a detection limit of 0.25nM. This strategy is simple, sensitive and selective with the minimal reagents and working steps, thereby holds great potential for Pb2+ detection in real environmental sample analysis.
Language eng
DOI 10.1016/j.snb.2015.08.046
Field of Research 030302 Nanochemistry and Supramolecular Chemistry
0301 Analytical Chemistry
0912 Materials Engineering
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID ARC DP130101714
Copyright notice ©2016, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080025

Connect to link resolver
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 25 times in TR Web of Science
Scopus Citation Count Cited 24 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 456 Abstract Views, 3 File Downloads  -  Detailed Statistics
Created: Thu, 03 Dec 2015, 10:16:21 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.