Graphene nanodots encaged 3-D gold substrate as enzyme loading platform for the fabrication of high performance biosensors

Wang, Jianmei, Zhu, Huihui, Xu, Yuanhong, Yang, Wenrong, Liu, Ao, Shan, Fukai, Cao, Mengmei and Liu, Jingquan 2015, Graphene nanodots encaged 3-D gold substrate as enzyme loading platform for the fabrication of high performance biosensors, Sensors and actuators, b: chemical, vol. 220, pp. 1186-1195, doi: 10.1016/j.snb.2015.06.044.

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Title Graphene nanodots encaged 3-D gold substrate as enzyme loading platform for the fabrication of high performance biosensors
Author(s) Wang, Jianmei
Zhu, Huihui
Xu, Yuanhong
Yang, WenrongORCID iD for Yang, Wenrong orcid.org/0000-0001-8815-1951
Liu, Ao
Shan, Fukai
Cao, Mengmei
Liu, Jingquan
Journal name Sensors and actuators, b: chemical
Volume number 220
Start page 1186
End page 1195
Total pages 10
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-12
ISSN 0925-4005
Summary Herein, a uniform three-dimensional (3-D) graphene nanodots-encaged porous gold electrode was prepared via ion beam sputtering deposition (IBSD) and mild corrosion chemistry for efficient enzyme electrode fabrication. Enzymes, like glucose oxidase and catalase, were modified with pyrene functionalities and then loaded into the graphene nanodots encaged porous gold electrode via non-covalent π-π stacking interaction between pyrene and graphene. The fabricated enzyme electrodes showed profound reusability and repeatability, high sensitivity, inherent selectivity and enhanced detection range. As for glucose analysis a broad linear range from 0.05 to 100 mM was obtained and the linear range for hydrogen peroxide was 0.005 to 4 mM. Detection limits of 30 μM for glucose and 1 μM for hydrogen peroxide were achieved (S/N = 3), respectively. These electrodes can be applied to analyze the clinical samples with reliable results. The formation mechanism and 3-D structure of the porous electrode were investigated using high resolution transmission electron microscope (HRTEM), atomic force microscopy (AFM), scanning electron microscope (SEM) and electrochemical impedance spectroscopy (EIS). Most importantly, various other ideal biosensors can be fabricated using the same porous electrode and the same enzyme modification methodology.
Language eng
DOI 10.1016/j.snb.2015.06.044
Field of Research 030107 Sensor Technology (Chemical aspects)
0301 Analytical Chemistry
0912 Materials Engineering
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID ARC DP130101714
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080048

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