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Attenuated total reflectance fourier transform infrared spectroscopy: an analytical technique to understand therapeutic responses at the molecular level

Kalmodia, Sushma, Parameswaran, Sowmya, Yang, Wenrong, Barrow, Colin J. and Krishnakumar, Subramanian 2015, Attenuated total reflectance fourier transform infrared spectroscopy: an analytical technique to understand therapeutic responses at the molecular level, Scientific reports, vol. 5, Article number : 16649, pp. 1-14, doi: 10.1038/srep16649.

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Title Attenuated total reflectance fourier transform infrared spectroscopy: an analytical technique to understand therapeutic responses at the molecular level
Author(s) Kalmodia, Sushma
Parameswaran, Sowmya
Yang, WenrongORCID iD for Yang, Wenrong orcid.org/0000-0001-8815-1951
Barrow, Colin J.ORCID iD for Barrow, Colin J. orcid.org/0000-0002-2153-7267
Krishnakumar, Subramanian
Journal name Scientific reports
Volume number 5
Season Article number : 16649
Start page 1
End page 14
Total pages 14
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2015
ISSN 2045-2322
2045-2322
Keyword(s) Cancer treatment
Molecular interactions
FTIR spectroscopy
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
FTIR MICROSPECTROSCOPY
IR-MICROSPECTROSCOPY
GOLD NANOPARTICLES
CELL-DEATH
IN-VIVO
CANCER
TISSUES
SURFACE
GENE
P53
Summary Rapid monitoring of the response to treatment in cancer patients is essential to predict the outcome of the therapeutic regimen early in the course of the treatment. The conventional methods are laborious, time-consuming, subjective and lack the ability to study different biomolecules and their interactions, simultaneously. Since; mechanisms of cancer and its response to therapy is dependent on molecular interactions and not on single biomolecules, an assay capable of studying molecular interactions as a whole, is preferred. Fourier Transform Infrared (FTIR) spectroscopy has become a popular technique in the field of cancer therapy with an ability to elucidate molecular interactions. The aim of this study, was to explore the utility of the FTIR technique along with multivariate analysis to understand whether the method has the resolution to identify the differences in the mechanism of therapeutic response. Towards achieving the aim, we utilized the mouse xenograft model of retinoblastoma and nanoparticle mediated targeted therapy. The results indicate that the mechanism underlying the response differed between the treated and untreated group which can be elucidated by unique spectral signatures generated by each group. The study establishes the efficiency of non-invasive, label-free and rapid FTIR method in assessing the interactions of nanoparticles with cellular macromolecules towards monitoring the response to cancer therapeutics.
Language eng
DOI 10.1038/srep16649
Field of Research 030105 Instrumental Methods (excl Immunological and Bioassay Methods)
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID ARC DP130101714
Copyright notice ©2015, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080052

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.