Oral administration of encapsulated bovine lactoferrin protein nanocapsules against intracellular parasite Toxoplasma gondii

Anand, Namrata, Sehgal, Rakesh, Kanwar, Rupinder Kaur, Dubey, Mohan Lal, Vasishta, Rakesh Kumar and Kanwar, Jagat Rakesh 2015, Oral administration of encapsulated bovine lactoferrin protein nanocapsules against intracellular parasite Toxoplasma gondii, International journal of nanomedicine, vol. 10, pp. 6355-6369, doi: 10.2147/IJN.S85286.

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Title Oral administration of encapsulated bovine lactoferrin protein nanocapsules against intracellular parasite Toxoplasma gondii
Author(s) Anand, Namrata
Sehgal, Rakesh
Kanwar, Rupinder Kaur
Dubey, Mohan Lal
Vasishta, Rakesh Kumar
Kanwar, Jagat RakeshORCID iD for Kanwar, Jagat Rakesh orcid.org/0000-0003-3728-9568
Journal name International journal of nanomedicine
Volume number 10
Start page 6355
End page 6369
Total pages 15
Publisher Dove Medical Press
Place of publication Auckland, New Zealand
Publication date 2015
ISSN 1178-2013
Keyword(s) Toxoplasma gondii
ceramic nanocapsules and reactive oxygen species
cytokines
immunoreactivity
nanocapsules
oral delivery
parasite load
Science & Technology
Life Sciences & Biomedicine
Nanoscience & Nanotechnology
Pharmacology & Pharmacy
Science & Technology - Other Topics
IRON-SATURATED LACTOFERRIN
NANOCARRIERS
CALCIUM
MECHANISM
DELIVERY
CELLS
Summary Toxoplasma gondii is a deadly intracellular parasite known to reside in every nucleated cell and known to cause severe complications in immunocompromised host. Standard drugs are cost effective and cause side effects, therefore, there is a necessity for a new drug molecule with immunomodulatory potential. Lactoferrin (Lf) is a natural milk protein, which has shown antimicrobial properties in its nanoformulation using alginate chitosan calcium phosphate bovine lactoferrin nanocapsules (AEC-CCo-CP-bLf-NCs). The present study was aimed to analyze and compare the effect of bovine Lf (bLf) in its native as well as nanoformulation (AEC-CCo-CP-bLf-NC) against coccidian parasite T. gondii. In vitro analysis has shown a significant increase in nitric oxide production and low parasitemia in in vitro cell culture model. In vivo BALB/c mice model have been used to develop human toxoplasmosis model. After treatment with NCs it has substantially increased the bioavailability of the protein and showed comparatively increased levels of reactive oxygen species, nitric oxide production, and Th1 cytokine which helped in parasite clearance. The mechanism of action of NCs has been clarified by immunoreactivity analysis, which showed accumulation of Lf in macrophages of various visceral organs, which is the site of parasite multiplication. Effect of NCs has significantly decreased (P<0.05) the parasite load in various organs and helped survival of mice till day 25 postinfection. Fe metabolism inside the mice has been found to be maintained even after administration of mono form of Lf, this indicates novelty of Lf protein. From the present study we concluded that nanoformulation did not reduce the therapeutic potential of Lf protein; however, nanoformulation has enhanced the stability of the protein and shown anti-toxoplasmal activity. Our study presents for the first time nanoformulation of Lf protein against Toxoplasma, which has advantages over the standard drug therapy without any side effects.
Language eng
DOI 10.2147/IJN.S85286
Field of Research 1007 Nanotechnology
1115 Pharmacology And Pharmaceutical Sciences
Socio Economic Objective 920299 Health and Support Services not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Dove Medical Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080174

Document type: Journal Article
Collection: School of Medicine
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