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Quick chip assay using locked nucleic acid modified epithelial cell adhesion molecule and nucleolin aptamers for the capture of circulating tumor cells

Maremanda, Nihal G., Roy, Kislay, Kanwar, Rupinder K., Shyamsundar, Vidyarani, Ramshankar,Vijayalakshmi, Krishnamurthy, Arvind, Krishnakumar, Subramanian and Kanwar, Jagat R. 2015, Quick chip assay using locked nucleic acid modified epithelial cell adhesion molecule and nucleolin aptamers for the capture of circulating tumor cells, Biomicrofluidics, vol. 9, no. 5, pp. 1-20, doi: 10.1063/1.4930983.

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Title Quick chip assay using locked nucleic acid modified epithelial cell adhesion molecule and nucleolin aptamers for the capture of circulating tumor cells
Author(s) Maremanda, Nihal G.
Roy, Kislay
Kanwar, Rupinder K.
Shyamsundar, Vidyarani
Ramshankar,Vijayalakshmi
Krishnamurthy, Arvind
Krishnakumar, Subramanian
Kanwar, Jagat R.ORCID iD for Kanwar, Jagat R. orcid.org/0000-0003-3728-9568
Journal name Biomicrofluidics
Volume number 9
Issue number 5
Start page 1
End page 20
Total pages 20
Publisher American Institute of Physics (AIP)
Place of publication Maryland, United States
Publication date 2015-09
ISSN 1932-1058
Summary The role of circulating tumor cells (CTCs) in disease diagnosis, prognosis, monitoring of the therapeutic efficacy, and clinical decision making is immense and has attracted tremendous focus in the last decade. We designed and fabricated simple, flat channel microfluidic devices polydimethylsiloxane (PDMS based) functionalized with locked nucleic acid (LNA) modified aptamers (targeting epithelial cell adhesion molecule (EpCAM) and nucleolin expression) for quick and efficient capture of CTCs and cancer cells. With optimized flow rates (10 μl/min), it was revealed that the aptamer modified devices offered reusability for up to six times while retaining optimal capture efficiency (>90%) and specificity. High capture sensitivity (92%) and specificity (100%) was observed in whole blood samples spiked with Caco-2 cells (10-100 cells/ml). Analysis of blood samples obtained from 25 head and neck cancer patients on the EpCAM LNA aptamer functionalized chip revealed that an average count of 5 ± 3 CTCs/ml of blood were captured from 22/25 samples (88%). EpCAM intracellular domain (EpICD) immunohistochemistry on 9 oral squamous cell carcinomas showed the EpICD positivity in the tumor cells, confirming the EpCAM expression in CTCs from head and neck cancers. These microfluidic devices also maintained viability for in vitro culture and characterization. Use of LNA modified aptamers provided added benefits in terms of cost effectiveness due to increased reusability and sustainability of the devices. Our results present a robust, quick, and efficient CTC capture platform with the use of simple PDMS based devices that are easy to fabricate at low cost and have an immense potential in cancer diagnosis, prognosis, and therapeutic planning.
Language eng
DOI 10.1063/1.4930983
Field of Research 111799 Public Health and Health Services not elsewhere classified
0915 Interdisciplinary Engineering
1007 Nanotechnology
0203 Classical Physics
Socio Economic Objective 920299 Health and Support Services not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2015, American Institute of Physics (AIP)
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080175

Document type: Journal Article
Collections: School of Medicine
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Created: Wed, 09 Dec 2015, 14:01:49 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.