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Vitamin C and E supplementation prevents some of the cellular adaptations to endurance-training in humans

Morrison, Dale, Hughes, Jed, Della Gatta, Paul A., Mason, Shaun, Lamon, Severine, Russell, Aaron P. and Wadley, Glenn D. 2015, Vitamin C and E supplementation prevents some of the cellular adaptations to endurance-training in humans, Free radical biology and medicine, vol. 89, pp. 852-862, doi: 10.1016/j.freeradbiomed.2015.10.412.

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Title Vitamin C and E supplementation prevents some of the cellular adaptations to endurance-training in humans
Author(s) Morrison, Dale
Hughes, Jed
Della Gatta, Paul A.ORCID iD for Della Gatta, Paul A. orcid.org/0000-0003-2231-8370
Mason, ShaunORCID iD for Mason, Shaun orcid.org/0000-0002-6138-2239
Lamon, SeverineORCID iD for Lamon, Severine orcid.org/0000-0002-3271-6551
Russell, Aaron P.ORCID iD for Russell, Aaron P. orcid.org/0000-0002-7323-9501
Wadley, Glenn D.ORCID iD for Wadley, Glenn D. orcid.org/0000-0002-6617-4359
Journal name Free radical biology and medicine
Volume number 89
Start page 852
End page 862
Total pages 11
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-12
ISSN 1873-4596
Keyword(s) Antioxidants
Endurance exercise
Mitochondrial biogenesis
Oxidative stress
Reactive oxygen species
Vitamin C
Vitamin E
Summary BACKGROUND: It is clear that reactive oxygen species (ROS) produced during skeletal muscle contraction have a regulatory role in skeletal muscle adaptation to endurance exercise. However, there is much controversy in the literature regarding whether attenuation of ROS by antioxidant supplementation can prevent these cellular adaptations. Therefore, the aim of this study was to determine whether vitamin C and E supplementation attenuates performance and cellular adaptations following acute endurance exercise and endurance training. METHODS: A double-blinded, placebo-controlled randomized control trial was conducted in eleven healthy young males. Participants were matched for peak oxygen consumption (VO2peak) and randomly allocated to placebo or antioxidant (vitamin C (2×500mg/day) and E (400IU/day)) groups. Following a four-week supplement loading period, participants completed acute exercise (10×4min cycling at 90% VO2peak, 2min active recovery). Vastus lateralis muscle samples were collected pre-, immediately-post- and 3h-post-exercise. Participants then completed four weeks of training (3 days/week) using the aforementioned exercise protocol while continuing supplementation. Following exercise training, participants again completed an acute exercise bout with muscle biopsies. RESULTS: Acute exercise tended to increase skeletal muscle oxidative stress as measured by oxidized glutathione (GSSG) (P=0.058) and F2-isoprostanes (P=0.056), with no significant effect of supplementation. Acute exercise significantly increased mRNA levels of peroxisome proliferator-activated receptor gamma coactivator 1α (PGC-1α), mitochondrial transcription factor A (TFAM) and PGC related coactivator (PRC), with no effect of supplementation. Following endurance training, supplementation did not prevent significantly increased VO2peak, skeletal muscle levels of citrate synthase activity or mRNA or protein abundance of cytochrome oxidase subunit 4 (COX IV) (P<0.05). However, following training, vitamin C and E supplementation significantly attenuated increased skeletal muscle superoxide dismutase (SOD) activity and protein abundance of SOD2 and TFAM. CONCLUSION: Following acute exercise, supplementation with vitamin C and E did not attenuate skeletal muscle oxidative stress or increased gene expression of mitochondrial biogenesis markers. However, supplementation attenuated some (SOD, TFAM) of the increased skeletal muscle adaptations following training in healthy young men.
Language eng
DOI 10.1016/j.freeradbiomed.2015.10.412
Field of Research 110604 Sports Medicine
110602 Exercise Physiology
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080233

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