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Parametric investigation of batch adsorption of proteins onto polymeric particles

Tan, Melvin X.L., Agyei, Dominic, Pan, Sharadwata and Danquah, Michael K 2015, Parametric investigation of batch adsorption of proteins onto polymeric particles, Current pharmaceutical biotechnology, vol. 16, no. 9, pp. 816-822, doi: 10.2174/1389201016666150629102624.

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Title Parametric investigation of batch adsorption of proteins onto polymeric particles
Author(s) Tan, Melvin X.L.
Agyei, DominicORCID iD for Agyei, Dominic orcid.org/0000-0003-2280-4096
Pan, Sharadwata
Danquah, Michael K
Journal name Current pharmaceutical biotechnology
Volume number 16
Issue number 9
Start page 816
End page 822
Total pages 7
Publisher Bentham Science Publishers
Place of publication Bussum, The Netherlands
Publication date 2015-06-28
ISSN 1873-4316
Keyword(s) Proteins, batch adsorption, liquid-solid mass transfer
Summary Background: Effective bimolecular adsorption of proteins onto solid matrices is characterized by in-depth understanding of the biophysical features essential to optimize the adsorption performance. Results: The adsorption of bovine serum albumin (BSA) onto anion-exchange Q-sepharose solid particulate support was investigated in batch adsorption experiments. Adsorption kinetics and isotherms were developed as a function of key industrially relevant parameters such as polymer loading, stirring speed, buffer pH, protein concentration and the state of protein dispersion (solid/aqueous) in order to optimize binding performance and adsorption capacity. Experimental results showed that the first order rate constant is higher at higher stirring speed, higher polymer loading, and under alkaline conditions, with a corresponding increase in equilibrium adsorption capacity. Increasing the stirring speed and using aqueous dispersion protein system increased the adsorption rate, but the maximum protein adsorption was unaffected. Regardless of the stirring speed, the adsorption capacity of the polymer was 2.8 mg/ml. However, doubling the polymer loading increased the adsorption capacity to 9.4 mg/ml. Conclusions: The result demonstrates that there exists a minimum amount of polymer loading required to achieve maximum protein adsorption capacity under specific process conditions.
Language eng
DOI 10.2174/1389201016666150629102624
Field of Research 1004 Medical Biotechnology
1115 Pharmacology And Pharmaceutical Sciences
090404 Membrane and Separation Technologies
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Bentham Science Publishers
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080272

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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