A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation

Pharo, Elizabeth A., Cane, Kylie N., McCoey, Julia, Buckle, Ashley M., Oosthuizen, W.H., Guinet, Christophe and Arnould, John P.Y. 2016, A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation, Gene, vol. 578, no. 1, pp. 7-16, doi: 10.1016/j.gene.2015.11.042.

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Title A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation
Author(s) Pharo, Elizabeth A.
Cane, Kylie N.
McCoey, Julia
Buckle, Ashley M.
Oosthuizen, W.H.
Guinet, Christophe
Arnould, John P.Y.ORCID iD for Arnould, John P.Y. orcid.org/0000-0003-1124-9330
Journal name Gene
Volume number 578
Issue number 1
Start page 7
End page 16
Total pages 10
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-03
ISSN 1879-0038
Keyword(s) BPTI
Summary The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel) and Perissodactyla (white rhinoceros). Unexpectedly, Weddell seal CTI was predicted to be a pseudogene. Cape fur seal CTI was expressed in the mammary gland of a pregnant multiparous seal, but not in a seal in its first pregnancy. While bovine CTI is expressed for 24-48h postpartum (pp) and secreted in colostrum only, Cape fur seal CTI was detected for at least 2-3months pp while the mother was suckling its young on-shore. Furthermore, CTI was expressed in the mammary gland of only one of the lactating seals that was foraging at-sea. The expression of β-casein (CSN2) and β-lactoglobulin II (LGB2), but not CTI in the second lactating seal foraging at-sea suggested that CTI may be intermittently expressed during lactation. Cape fur seal and walrus CTI encode putative small, secreted, N-glycosylated proteins with a single Kunitz/bovine pancreatic trypsin inhibitor (BPTI) domain indicative of serine protease inhibition. Mature Cape fur seal CTI shares 92% sequence identity with Pacific walrus CTI, but only 35% identity with BPTI. Structural homology modelling of Cape fur seal CTI and Pacific walrus trypsin based on the model of the second Kunitz domain of human tissue factor pathway inhibitor (TFPI) and porcine trypsin (Protein Data Bank: 1TFX) confirmed that CTI inhibits trypsin in a canonical fashion. Therefore, pinniped CTI may be critical for preventing the proteolytic degradation of immunoglobulins that are passively transferred from mother to young via colostrum and milk.
Language eng
DOI 10.1016/j.gene.2015.11.042
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
060205 Marine and Estuarine Ecology (incl Marine Ichthyology)
060407 Genome Structure and Regulation
0604 Genetics
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080469

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