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Visualization and quantitation of GLUT4 translocation in human skeletal muscle following glucose ingestion and exercise

Bradley, Helen, Shaw, Christopher S., Bendtsen, Claus, Worthington, Philip L., Wilson, Oliver J., Strauss, Juliette A., Wallis, Gareth A., Turner, Alice M. and Wagenmakers, Anton J.M. 2015, Visualization and quantitation of GLUT4 translocation in human skeletal muscle following glucose ingestion and exercise, Physiological reports, vol. 3, no. 5, Article number : e12375, pp. 1-11, doi: 10.14814/phy2.12375.

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Title Visualization and quantitation of GLUT4 translocation in human skeletal muscle following glucose ingestion and exercise
Author(s) Bradley, Helen
Shaw, Christopher S.
Bendtsen, Claus
Worthington, Philip L.
Wilson, Oliver J.
Strauss, Juliette A.
Wallis, Gareth A.
Turner, Alice M.
Wagenmakers, Anton J.M.
Journal name Physiological reports
Volume number 3
Issue number 5
Season Article number : e12375
Start page 1
End page 11
Total pages 11
Publisher American Physiological Society
Place of publication Bethesda, M.D.
Publication date 2015-05-12
ISSN 2051-817X
Keyword(s) Exercise
Glucose ingestion
GLUT4 translocation
Skeletal muscle
Summary Insulin- and contraction-stimulated increases in glucose uptake into skeletal
muscle occur in part as a result of the translocation of glucose transporter 4
(GLUT4) from intracellular stores to the plasma membrane (PM). This study
aimed to use immunofluorescence microscopy in human skeletal muscle to
quantify GLUT4 redistribution from intracellular stores to the PM in response
to glucose feeding and exercise. Percutaneous muscle biopsy samples were
taken from the m. vastus lateralis of ten insulin-sensitive men in the basal
state and following 30 min of cycling exercise (65% VO2 max). Muscle biopsy
samples were also taken from a second cohort of ten age-, BMI- and VO2 maxmatched insulin-sensitive men in the basal state and 30 and 60 min following
glucose feeding (75 g glucose). GLUT4 and dystrophin colocalization, measured
using the Pearson’s correlation coefficient, was increased following
30 min of cycling exercise (baseline r = 0.47 0.01; post exercise
r = 0.58 0.02; P < 0.001) and 30 min after glucose ingestion (baseline
r = 0.42 0.02; 30 min r = 0.46 0.02; P < 0.05). Large and small GLUT4
clusters were partially depleted following 30 min cycling exercise, but not
30 min after glucose feeding. This study has, for the first time, used immunofluorescence microscopy in human skeletal muscle to quantify increases in
GLUT4 and dystrophin colocalization and depletion of GLUT4 from large
and smaller clusters as evidence of net GLUT4 translocation to the PM.
Language eng
DOI 10.14814/phy2.12375
Field of Research 110699 Human Movement and Sports Science not elsewhere classified
Socio Economic Objective 920116 Skeletal System and Disorders (incl. Arthritis)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080491

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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