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Forms of n-3 (ALA, C18:3n-3 or DHA, C22:6n-3) fatty acids affect carcass yield, blood lipids, muscle n-3 fatty acids and liver gene expression in lambs

Ponnampalam, Eric N., Lewandowski, Paul A., Fahri, Fahri T., Burnett, Viv F., Dunshea, Frank R., Plozza, Tim and Jacobs, Joe L. 2015, Forms of n-3 (ALA, C18:3n-3 or DHA, C22:6n-3) fatty acids affect carcass yield, blood lipids, muscle n-3 fatty acids and liver gene expression in lambs, Lipids, vol. 50, no. 11, pp. 1133-1143, doi: 10.1007/s11745-015-4070-4.

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Title Forms of n-3 (ALA, C18:3n-3 or DHA, C22:6n-3) fatty acids affect carcass yield, blood lipids, muscle n-3 fatty acids and liver gene expression in lambs
Author(s) Ponnampalam, Eric N.
Lewandowski, Paul A.
Fahri, Fahri T.
Burnett, Viv F.
Dunshea, Frank R.
Plozza, Tim
Jacobs, Joe L.
Journal name Lipids
Volume number 50
Issue number 11
Start page 1133
End page 1143
Total pages 11
Publisher Springer
Place of publication Berlin, Germany
Publication date 2015-11
ISSN 1558-9307
Keyword(s) Algae
Blood insulin
Blood lipids
Carcass yield
Flaxseed
Liver gene expression
Muscle fatty acids
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Nutrition & Dietetics
INSULIN-RESISTANCE
ENERGY-BALANCE
IN-VIVO
METHANE
MEAT
TRIGLYCERIDE
SYSTEMS
OMEGA-3-FATTY-ACIDS
BIOAVAILABILITY
Summary The effects of supplementing diets with n-3 alpha-linolenic acid (ALA) and docosahexaenoic acid (DHA) on plasma metabolites, carcass yield, muscle n-3 fatty acids and liver messenger RNA (mRNA) in lambs were investigated. Lambs (n = 120) were stratified to 12 groups based on body weight (35 ± 3.1 kg), and within groups randomly allocated to four dietary treatments: basal diet (BAS), BAS with 10.7 % flaxseed supplement (Flax), BAS with 1.8 % algae supplement (DHA), BAS with Flax and DHA (FlaxDHA). Lambs were fed for 56 days. Blood samples were collected on day 0 and day 56, and plasma analysed for insulin and lipids. Lambs were slaughtered, and carcass traits measured. At 30 min and 24 h, liver and muscle samples, respectively, were collected for determination of mRNA (FADS1, FADS2, CPT1A, ACOX1) and fatty acid composition. Lambs fed Flax had higher plasma triacylglycerol, body weight, body fat and carcass yield compared with the BAS group (P < 0.001). DHA supplementation increased carcass yield and muscle DHA while lowering plasma insulin compared with the BAS diet (P < 0.01). Flax treatment increased (P < 0.001) muscle ALA concentration, while DHA treatment increased (P < 0.001) muscle DHA concentration. Liver mRNA FADS2 was higher and CPT1A lower in the DHA group (P < 0.05). The FlaxDHA diet had additive effects, including higher FADS1 and ACOX1 mRNA than for the Flax or DHA diet. In summary, supplementation with ALA or DHA modulated plasma metabolites, muscle DHA, body fat and liver gene expression differently.
Language eng
DOI 10.1007/s11745-015-4070-4
Field of Research 11 Medical And Health Sciences
07 Agricultural And Veterinary Sciences
09 Engineering
110104 Medical Biochemistry: Lipids
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080496

Document type: Journal Article
Collection: Molecular and Medical Research
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