Hormonal regulation of platypus Beta-lactoglobulin and monotreme lactation protein genes

Enjapoori, Ashwantha Kumar, Lefèvre, Christophe M, Nicholas, Kevin R and Sharp, Julie A 2017, Hormonal regulation of platypus Beta-lactoglobulin and monotreme lactation protein genes, General and comparative endocrinology, vol. 242, pp. 38-48, doi: 10.1016/j.ygcen.2015.12.005.

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Title Hormonal regulation of platypus Beta-lactoglobulin and monotreme lactation protein genes
Author(s) Enjapoori, Ashwantha Kumar
Lefèvre, Christophe M
Nicholas, Kevin R
Sharp, Julie AORCID iD for Sharp, Julie A orcid.org/0000-0002-4481-5223
Journal name General and comparative endocrinology
Volume number 242
Start page 38
End page 48
Total pages 11
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2017-02-01
ISSN 1095-6840
Keyword(s) Platypus
Hormonal regulation
Milk protein gene expression
Monotreme lactation protein
Platypus Beta-lactoglobulin gene
Summary Endocrine regulation of milk protein gene expression in marsupials and eutherians is well studied. However, the evolution of this complex regulation that began with monotremes is unknown. Monotremes represent the oldest lineage of extant mammals and the endocrine regulation of lactation in these mammals has not been investigated. Here we characterised the proximal promoter and hormonal regulation of two platypus milk protein genes, Beta-lactoglobulin (BLG), a whey protein and monotreme lactation protein (MLP), a monotreme specific milk protein, using in vitro reporter assays and a bovine mammary epithelial cell line (BME-UV1). Insulin and dexamethasone alone provided partial induction of MLP, while the combination of insulin, dexamethasone and prolactin was required for maximal induction. Partial induction of BLG was achieved by insulin, dexamethasone and prolactin alone, with maximal induction using all three hormones. Platypus MLP and BLG core promoter regions comprised transcription factor binding sites (e.g. STAT5, NF-1 and C/EBPα) that were conserved in marsupial and eutherian lineages that regulate caseins and whey protein gene expression. Our analysis suggests that insulin, dexamethasone and/or prolactin alone can regulate the platypus MLP and BLG gene expression, unlike those of therian lineage. The induction of platypus milk protein genes by lactogenic hormones suggests they originated before the divergence of marsupial and eutherians.
Language eng
DOI 10.1016/j.ygcen.2015.12.005
Field of Research 0608 Zoology
0606 Physiology
0707 Veterinary Sciences
Socio Economic Objective 839901 Animal Welfare
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080503

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