Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns Pravadali-Cekic, Sercan, Kocic, Danijela, Conlan, Xavier and Shalliker, R Andrew 2015, Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns, Journal of liquid chromatography and related technologies, vol. 38, no. 19, pp. 1753-1758, doi: 10.1080/10826076.2015.1105256. Attached Files Name Description MIMEType Size Downloads Title Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns Author(s) Pravadali-Cekic, Sercan Kocic, Danijela Conlan, Xavier orcid.org/0000-0003-0829-0551 Shalliker, R Andrew Journal name Journal of liquid chromatography and related technologies Volume number 38 Issue number 19 Start page 1753 End page 1758 Total pages 6 Publisher Taylor & Francis Place of publication London, Eng. Publication date 2015 ISSN 1082-6076 1520-572X Keyword(s) Active flow technology DPPH center dot High performance liquid chromatography Multiplexed detection Natural products Parallel segmented flow Summary Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH• for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive. Language eng DOI 10.1080/10826076.2015.1105256 Field of Research 030108 Separation Science 0301 Analytical Chemistry 0399 Other Chemical Sciences Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2015, Taylor & Francis Persistent URL http://hdl.handle.net/10536/DRO/DU:30080540 Document type: Journal Article Collections: School of Life and Environmental Sciences Centre for Biotechnology, Chemistry and Systems Biology Related Links Link Description Connect to published version Go to link with your DU access privileges   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Thu, 07 Jan 2016, 10:31:34 EST Thu, 07 Jan 2016, 10:32:24 EST Fri, 08 Jan 2016, 04:02:02 EST Fri, 08 Jan 2016, 05:11:59 EST Wed, 08 Jun 2016, 15:14:55 EST Wed, 08 Jun 2016, 15:20:50 EST Thu, 16 Jun 2016, 16:09:06 EST Wed, 07 Sep 2016, 11:02:04 EST Wed, 01 Mar 2017, 12:51:04 EST Filtered Full Citation counts:   Cited 1 times in TR Web of Science Cited 1 times in Scopus Search Google Scholar Access Statistics: 70 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Thu, 07 Jan 2016, 10:32:24 EST

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