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Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns

Pravadali-Cekic, Sercan, Kocic, Danijela, Conlan, Xavier and Shalliker, R Andrew 2015, Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns, Journal of liquid chromatography and related technologies, vol. 38, no. 19, pp. 1753-1758, doi: 10.1080/10826076.2015.1105256.

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Title Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns
Author(s) Pravadali-Cekic, Sercan
Kocic, Danijela
Conlan, XavierORCID iD for Conlan, Xavier orcid.org/0000-0003-0829-0551
Shalliker, R Andrew
Journal name Journal of liquid chromatography and related technologies
Volume number 38
Issue number 19
Start page 1753
End page 1758
Total pages 6
Publisher Taylor & Francis
Place of publication London, Eng.
Publication date 2015
ISSN 1082-6076
1520-572X
Keyword(s) Active flow technology
DPPH center dot
High performance liquid chromatography
Multiplexed detection
Natural products
Parallel segmented flow
Summary Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH• for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive.
Language eng
DOI 10.1080/10826076.2015.1105256
Field of Research 030108 Separation Science
0301 Analytical Chemistry
0399 Other Chemical Sciences
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Taylor & Francis
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080540

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