Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns
Pravadali-Cekic, Sercan, Kocic, Danijela, Conlan, Xavier and Shalliker, R Andrew 2015, Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns, Journal of liquid chromatography and related technologies, vol. 38, no. 19, pp. 1753-1758, doi: 10.1080/10826076.2015.1105256.
Attached Files
Name
Description
MIMEType
Size
Downloads
Title
Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns
Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH• for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive.
Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.