You are not logged in.

Bead milling for lipid recovery from thraustochytrid cells and selective hydrolysis of Schizochytrium DT3 oil using lipase

Byreddy, Avinesh R., Barrow, Colin J. and Puri, Munish 2016, Bead milling for lipid recovery from thraustochytrid cells and selective hydrolysis of Schizochytrium DT3 oil using lipase, Bioresource technology, vol. 200, pp. 464-469, doi: 10.1016/j.biortech.2015.10.019.

Attached Files
Name Description MIMEType Size Downloads

Title Bead milling for lipid recovery from thraustochytrid cells and selective hydrolysis of Schizochytrium DT3 oil using lipase
Author(s) Byreddy, Avinesh R.
Barrow, Colin J.ORCID iD for Barrow, Colin J. orcid.org/0000-0002-2153-7267
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Journal name Bioresource technology
Volume number 200
Start page 464
End page 469
Total pages 6
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-01
ISSN 1873-2976
Keyword(s) Bioactives
Candida rugosa
Cell disruption
Enzymes
Schizochytrium
Science & Technology
Life Sciences & Biomedicine
Technology
Agricultural Engineering
Biotechnology & Applied Microbiology
Energy & Fuels
Agriculture
POLYUNSATURATED FATTY-ACIDS
CANDIDA-RUGOSA LIPASE
BIODIESEL PRODUCTION
CHLORELLA-VULGARIS
CARBON-SOURCES
DISRUPTION
MICROALGAE
EXTRACTION
OMEGA-3
DISINTEGRATION
Summary Marine microalgae present a renewable alternative source for sustainable production of omega-3 fatty acids, as compared to conventional sources such as krill oil and fish oil. In this study, we optimised a method for lipid extraction from marine thraustochytrids using a bead mill and enzymatic concentration of omega-3 fatty acids from the thraustochytrid oil. The optimised lipid extraction conditions were, bead size 0.4-0.6μm, 4500rpm, 4min of processing time at 5g biomass concentration. The maximum lipid yield (% dry weight basis) achieved at optimum conditions were 40.5% for Schizochytrium sp. S31 (ATCC) and 49.4% for Schizochytrium sp. DT3 (in-house isolate). DT3 oil contained 39.8% docosahexaenoic acid (DHA) as a percentage of lipid, a higher DHA percentage than S31. Partial hydrolysis of DT3 oil using Candida rugosa lipase was performed to enrich omega-3 polyunsaturated fatty acids (PUFAs) in the glyceride portion. Total omega-3 fatty acid content was increased to 88.7%.
Language eng
DOI 10.1016/j.biortech.2015.10.019
Field of Research 060107 Enzymes
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080586

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 6 times in TR Web of Science
Scopus Citation Count Cited 6 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 213 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Mon, 11 Jan 2016, 14:52:46 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.