Postprandial plasma phospholipids in men are influenced by the source of dietary fat Meikle, Peter J., Barlow, Christopher K., Mellett, Natalie A., Mundra, Piyushkumar A., Bonham, Maxine P., Larsen, Amy, Cameron-Smith, David, Sinclair, Andrew, Nestel, Paul J. and Wong, Gerard 2015, Postprandial plasma phospholipids in men are influenced by the source of dietary fat, Journal of nutrition, vol. 145, no. 9, pp. 2012-2018, doi: 10.3945/jn.115.210104. Attached Files Name Description MIMEType Size Downloads Title Postprandial plasma phospholipids in men are influenced by the source of dietary fat Author(s) Meikle, Peter J. Barlow, Christopher K. Mellett, Natalie A. Mundra, Piyushkumar A. Bonham, Maxine P. Larsen, Amy Cameron-Smith, David Sinclair, Andrew Nestel, Paul J. Wong, Gerard Journal name Journal of nutrition Volume number 145 Issue number 9 Start page 2012 End page 2018 Total pages 7 Publisher American Society for Nutrition Place of publication Rockville, Md Publication date 2015-07-15 ISSN 1541-6100 Keyword(s) dairy humans lipidomics mass spectrometry phospholipids soy Summary BACKGROUND: Postprandial lipemia represents a risk factor for chronic diseases, including type 2 diabetes. Little is known about the effect of dietary fat on the plasma lipidome in the postprandial period. OBJECTIVE: The objective of this study was to assess the effect of dairy fat and soy oil on circulating postprandial lipids in men. METHODS: Men (40-60 y old, nonsmokers; n = 16) were randomly assigned in a crossover design to consume 2 breakfast meals of dairy-based or soy oil-based foods. The changes in the plasma lipidome during the 4-h postprandial period were analyzed with electrospray ionization tandem mass spectrometry and included 316 lipid species in 23 classes and subclasses, representing sphingolipids, phospholipids, glycerolipids, and sterols. RESULTS: Nonparametric Friedman tests showed significant changes in multiple plasma lipid classes, subclasses, and species in the postprandial period after both dairy and soy meals. No difference was found in triglyceridemia after each meal. However, 6 endogenous lipid classes increased after dairy but decreased after soy (P < 0.05), including ether-linked phospholipids and plasmalogens and sphingomyelin (not present in soy), dihexosylceramide, and GM3 ganglioside. Phosphatidylcholine and phosphatidylinositol were not affected by the soy meal but were significantly elevated after the dairy meal (8.3% and 16%, respectively; P < 0.05). CONCLUSIONS: The changes in postprandial plasma phospholipids in men relate to the diet composition and the relative size of the endogenous phospholipid pools. Despite similar lipemic responses as measured by changes in triglyceride concentrations, the differential responses to dairy and soy meals derived through lipidomic analysis of phospholipids suggest differences in the metabolism of soybean oil and dairy fat. The increased concentrations of plasmalogens, with potential antioxidant capacity, in the postprandial period after dairy but not soy meals may represent a further important difference in the response to these sources of fat. The trial was registered at www.anzctr.org.au as ACTRN12610000562077. Language eng DOI 10.3945/jn.115.210104 Field of Research 111103 Nutritional Physiology 1111 Nutrition And Dietetics 0702 Animal Production 0908 Food Sciences Socio Economic Objective 920411 Nutrition HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2015, American Society for Nutrition Persistent URL http://hdl.handle.net/10536/DRO/DU:30080641 Document type: Journal Article Collections: Faculty of Health Molecular and Medical Research Related Links Link Description Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 11 Jan 2016, 08:50:10 EST Tue, 12 Jan 2016, 04:00:49 EST Wed, 13 Jan 2016, 05:14:19 EST Tue, 05 Jul 2016, 09:48:59 EST Tue, 05 Jul 2016, 09:59:12 EST Tue, 12 Jul 2016, 12:26:11 EST Thu, 08 Sep 2016, 10:21:36 EST Thu, 08 Sep 2016, 10:28:12 EST Thu, 08 Sep 2016, 10:29:00 EST Filtered Full Citation counts:   Cited 23 times in TR Web of Science Cited 22 times in Scopus Search Google Scholar Access Statistics: 240 Abstract Views, 3 File Downloads  -  Detailed Statistics Created: Mon, 11 Jan 2016, 08:50:10 EST

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