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Probing the biophysical interaction between Neocarzinostatin toxin and EpCAM RNA aptamer

Athyala, Prasanna Kumar, Kanwar, Jagat Rakesh, Alameen, Mohamed, Kanwar, Rupinder Kaur, Krishnakumar, Subramanian, Watson, Jon, Vetrivel, Umashankar and Narayanan, Janakiraman 2016, Probing the biophysical interaction between Neocarzinostatin toxin and EpCAM RNA aptamer, Biochemical and biophysical research communications, vol. 469, no. 2, pp. 257-262, doi: 10.1016/j.bbrc.2015.11.109.

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Title Probing the biophysical interaction between Neocarzinostatin toxin and EpCAM RNA aptamer
Author(s) Athyala, Prasanna Kumar
Kanwar, Jagat RakeshORCID iD for Kanwar, Jagat Rakesh orcid.org/0000-0003-3728-9568
Alameen, Mohamed
Kanwar, Rupinder Kaur
Krishnakumar, Subramanian
Watson, JonORCID iD for Watson, Jon orcid.org/0000-0002-2953-9806
Vetrivel, Umashankar
Narayanan, Janakiraman
Journal name Biochemical and biophysical research communications
Volume number 469
Issue number 2
Start page 257
End page 262
Total pages 6
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-01-08
ISSN 1090-2104
1090-2104
Keyword(s) CD spectroscopy
EpCAM aptamer
Insilico modeling
Neocarzinostatin
Antigens, Neoplasm
Aptamers, Nucleotide
Binding Sites
Cell Adhesion Molecules
Cytotoxins
Models, Chemical
Molecular Conformation
Molecular Docking Simulation
Protein Binding
Protein Interaction Mapping
Zinostatin
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Biophysics
APO-NEOCARZINOSTATIN
GRAPHICS PROCESSORS
SECONDARY STRUCTURE
ANTITUMOR PROTEIN
CRYSTAL-STRUCTURE
CHROMOPHORE
APONEOCARZINOSTATIN
STABILIZATION
APOPROTEIN
ENEDIYNE
Summary Neocarzinostatin (NCS) a potent DNA-damaging, anti-tumor toxin extracted from Streptomyces carzinostaticus that recognizes double-stranded DNA bulge and induces DNA damage. 2 Fluoro (2F) Modified EpCAM RNA aptamer is a 23-mer that targets EpCAM protein, expressed on the surface of epithelial tumor cells. Understanding the interaction between NCS and the ligand is important for carrying out the targeted tumor therapy. In this study, we have investigated the biophysical interactions between NCS and 2-fluro Modified EpCAM RNA aptamer using Circular Dichroism (CD) and Infra-Red (IR) spectroscopy. The aromatic amino acid residues spanning the β sheets of NCS are found to participate in intermolecular interactions with 2 F Modified EpCAM RNA aptamer. In-silico modeling and simulation studies corroborate with CD spectra data. Furthermore, it reinforces the involvement of C and D1 strand of NCS in intermolecular interactions with EpCAM RNA aptamer. This the first report on interactions involved in the stabilization of NCS-EpCAM aptamer complex and will aid in the development of therapeutic modalities towards targeted cancer therapy.
Language eng
DOI 10.1016/j.bbrc.2015.11.109
Field of Research 030405 Molecular Medicine
0304 Medicinal And Biomolecular Chemistry
0601 Biochemistry And Cell Biology
1101 Medical Biochemistry And Metabolomics
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080643

Document type: Journal Article
Collection: School of Medicine
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