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Biodegradable Eri silk nanoparticles as a delivery vehicle for bovine lactoferrin against MDA-MB-231 and MCF-7 breast cancer cells

Roy, Kislay, Patel, Yogesh S., Kanwar, Rupinder K., Rajkhowa, Rangam, Wang, Xungai and Kanwar, Jagat R. 2016, Biodegradable Eri silk nanoparticles as a delivery vehicle for bovine lactoferrin against MDA-MB-231 and MCF-7 breast cancer cells, International journal of nanomedicine, vol. 11, pp. 25-44, doi: 10.2147/IJN.S91810.

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Title Biodegradable Eri silk nanoparticles as a delivery vehicle for bovine lactoferrin against MDA-MB-231 and MCF-7 breast cancer cells
Author(s) Roy, Kislay
Patel, Yogesh S.
Kanwar, Rupinder K.
Rajkhowa, Rangam
Wang, Xungai
Kanwar, Jagat R.
Journal name International journal of nanomedicine
Volume number 11
Start page 25
End page 44
Total pages 20
Publisher Dove Medical Press
Place of publication Macclesfield, Eng.
Publication date 2016
ISSN 1178-2013
Keyword(s) apoptosis
bovine lactoferrin
breast cancer
epidermal growth factor receptor
silk nanoparticles
Summary This study used the Eri silk nanoparticles (NPs) for delivering apo-bovine lactoferrin (Apo-bLf) (~2% iron saturated) and Fe-bLf (100% iron saturated) in MDA-MB-231 and MCF-7 breast cancer cell lines. Apo-bLf and Fe-bLf-loaded Eri silk NPs with sizes between 200 and 300 nm (±10 nm) showed a significant internalization within 4 hours in MDA-MB-231 cells when compared to MCF-7 cells. The ex vivo loop assay with chitosan-coated Fe-bLf-loaded silk NPs was able to substantiate its future use in oral administration and showed the maximum absorption within 24 hours by ileum. Both Apo-bLf and Fe-bLf induced increase in expression of low-density lipoprotein receptor-related protein 1 and lactoferrin receptor in epidermal growth factor (EGFR)-positive MDA-MB-231 cells, while transferrin receptor (TfR) and TfR2 in MCF-7 cells facilitated the receptor-mediated endocytosis of NPs. Controlled and sustained release of both bLf from silk NPs was shown to induce more cancer-specific cytotoxicity in MDA-MB-231 and MCF-7 cells compared to normal MCF-10A cells. Due to higher degree of internalization, the extent of cytotoxicity and apoptosis was significantly higher in MDA-MB-231 (EGFR+) cells when compared to MCF-7 (EGFR-) cells. The expression of a prominent anticancer target, survivin, was found to be downregulated at both gene and protein levels. Taken together, all the observations suggest the potential use of Eri silk NPs as a delivery vehicle for an anti-cancer milk protein, and indicate bLf for the treatment of breast cancer.
Language eng
DOI 10.2147/IJN.S91810
Field of Research 1007 Nanotechnology
1115 Pharmacology And Pharmaceutical Sciences
100703 Nanobiotechnology
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution non-commercial licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080714

Document type: Journal Article
Collections: School of Medicine
Institute for Frontier Materials
Open Access Collection
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Created: Thu, 14 Jan 2016, 10:25:48 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.