Identification and topographical characterisation of microbial nanowires in Nostoc punctiforme

Sure, Sandeep, Torriero, Angel A.J., Gaur, Aditya, Li, Lu Hua, Chen, Ying, Tripathi, Chandrakant, Adholeya, Alok, Ackland, M. Leigh and Kochar, Mandira 2016, Identification and topographical characterisation of microbial nanowires in Nostoc punctiforme, Antonie Van Leeuwenhoek, vol. 109, no. 3, pp. 475-480, doi: 10.1007/s10482-015-0644-7.

Attached Files
Name Description MIMEType Size Downloads

Title Identification and topographical characterisation of microbial nanowires in Nostoc punctiforme
Formatted title Identification and topographical characterisation of microbial nanowires in Nostoc punctiforme
Author(s) Sure, Sandeep
Torriero, Angel A.J.ORCID iD for Torriero, Angel A.J. orcid.org/0000-0001-8616-365X
Gaur, Aditya
Li, Lu HuaORCID iD for Li, Lu Hua orcid.org/0000-0003-2435-5220
Chen, YingORCID iD for Chen, Ying orcid.org/0000-0002-7322-2224
Tripathi, Chandrakant
Adholeya, Alok
Ackland, M. LeighORCID iD for Ackland, M. Leigh orcid.org/0000-0002-7474-6556
Kochar, Mandira
Journal name Antonie Van Leeuwenhoek
Volume number 109
Issue number 3
Start page 475
End page 480
Total pages 6
Publisher Springer
Place of publication Cham, Switzerland
Publication date 2016-03
ISSN 0003-6072
1572-9699
Keyword(s) cyanobacteria
microbial nanowires
Nostoc punctiforme
pili-like structure
Summary Extracellular pili-like structures (PLS) produced by cyanobacteria have been poorly explored. We have done detailed topographical and electrical characterisation of PLS in Nostoc punctiforme PCC 73120 using transmission electron microscopy (TEM) and conductive atomic force microscopy (CAFM). TEM analysis showed that N. punctiforme produces two separate types of PLS differing in their length and diameter. The first type of PLS are 6-7.5 nm in diameter and 0.5-2 µm in length (short/thin PLS) while the second type of PLS are ~20-40 nm in diameter and more than 10 µm long (long/thick PLS). This is the first study to report long/thick PLS in N. punctiforme. Electrical characterisation of these two different PLS by CAFM showed that both are electrically conductive and can act as microbial nanowires. This is the first report to show two distinct PLS and also identifies microbial nanowires in N. punctiforme. This study paves the way for more detailed investigation of N. punctiforme nanowires and their potential role in cell physiology and symbiosis with plants.
Language eng
DOI 10.1007/s10482-015-0644-7
Field of Research 060101 Analytical Biochemistry
030102 Electroanalytical Chemistry
0605 Microbiology
1108 Medical Microbiology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080736

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in TR Web of Science
Scopus Citation Count Cited 4 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 360 Abstract Views, 2 File Downloads  -  Detailed Statistics
Created: Thu, 05 May 2016, 12:52:13 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.