Outlining a multidimensional approach for the analysis of coffee using HPLC

Pravadali, Sercan, Kocic, Danijela, Stevenson, Paul G and Shalliker, Andrew R 2015, Outlining a multidimensional approach for the analysis of coffee using HPLC, Journal of chromatography & separation techniques, vol. 6, no. 5, Article Number : 1000284, pp. 1-10, doi: 10.4172/2157-7064.1000284.

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Title Outlining a multidimensional approach for the analysis of coffee using HPLC
Author(s) Pravadali, Sercan
Kocic, Danijela
Stevenson, Paul GORCID iD for Stevenson, Paul G orcid.org/0000-0001-6780-6859
Shalliker, Andrew R
Journal name Journal of chromatography & separation techniques
Volume number 6
Issue number 5
Season Article Number : 1000284
Start page 1
End page 10
Total pages 10
Publisher Omics International
Place of publication Hyderabad, India
Publication date 2015
ISSN 2157-7064
Summary This study investigated approaches for the profiling of coffee using two multidimensional approaches: (1) a multi-detection process and (2) a multi-separation process employing HPLC. The first approach compared multidetection techniques of conventional High Performance Liquid Chromatography (HPLC) hyphenated with a detector (DPPH•, UV-Vis and MS), and multiplexed mode via HPLC with an Active Flow Technology (AFT) column in Parallel Segmented Flow (PSF) format with DPPH• detection, UV-Vis and MS running simultaneously. Multiplexed HPLCPSF enabled the determination of key chemical entities by reducing the data complexity of the sample whilst obtaining a greater degree of molecule-specific information within a fraction of the time it takes using conventional multi-detection processes. DPPH•, UV-Vis and MS (TIC) were multiplexed for the analysis of espresso coffee and decaffeinated espresso coffee. Up to 20 DPPH• peaks were detected for each sample, and with direct retention time peak matching, 70% of DPPH• peaks gave a UV-Vis response for the espresso coffee and 95% for the decaffeinated espresso coffee. The second approach involved the use of a two-dimensional (2D) HPLC system to expand the separation space and separation power for the analysis of coffee, focusing on the resolution and detection of coeluting and overlapping peaks, which was beyond the limits of conventional HPLC in resolving complex samples. The 2DHPLC analysis resulted with the detection of 176 peaks and a closer observation showed the presence of an additional 17 peaks in a cut section where in 1D mode only one peak was observed.
Language eng
DOI 10.4172/2157-7064.1000284
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C2 Other contribution to refereed journal
ERA Research output type C Journal article
Copyright notice ©2015, OMICS International
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080742

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