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Collagen type III and VI turnover in response to long-term immobilization

Sun, Shu, Henriksen, Kim, Karsdal, Morten A., Byrjalsen, Inger, Rittweger, Jorn, Armbrecht, Gabriele, Belavy, Daniel L., Felsenberg, Dieter and Nedergaard, Anders F. 2015, Collagen type III and VI turnover in response to long-term immobilization, PLoS One, vol. 10, no. 12, Article Number : e0144525, pp. 1-14, doi: 10.1371/journal.pone.0144525.

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Title Collagen type III and VI turnover in response to long-term immobilization
Author(s) Sun, Shu
Henriksen, Kim
Karsdal, Morten A.
Byrjalsen, Inger
Rittweger, Jorn
Armbrecht, Gabriele
Belavy, Daniel L.ORCID iD for Belavy, Daniel L. orcid.org/0000-0002-9307-832X
Felsenberg, Dieter
Nedergaard, Anders F.
Journal name PLoS One
Volume number 10
Issue number 12
Season Article Number : e0144525
Start page 1
End page 14
Total pages 14
Publisher Public Library of Science (PLoS)
Place of publication San Francisco, Calif.
Publication date 2015
ISSN 1932-6203
Keyword(s) Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
MUSCLE PROTEIN-SYNTHESIS
SKELETAL-MUSCLE
DISUSE ATROPHY
BED-REST
SEROLOGICAL BIOMARKERS
C5 DOMAIN
EXERCISE
EXPRESSION
MATRIX
MASS
Summary BACKGROUND: Muscle mass and function are perturbed by immobilization and remobilization. When muscle mass changes, the quality and quantity of the extracellular matrix protein, particularly the collagens, change with it. In this study, we investigated the temporal profile of three peptide biomarkers derived from turnover of collagen type III and type VI in a long-term immobilization and remobilization study. We also compared individual biomarker levels with Lean body Mass (LBM) and changes therein, hypothesizing that these biomarkers would be biomarkers of the remodeling processes associated with immobilization and/or remobilization. METHODS: In the Berlin bed rest study, 20 young men were recruited and randomly assigned to 8-week's strict bed rest with or without resistive vibration exercise countermeasure. We measured three neo-epitope ELISA kits in the serum samples of this study: Pro-C3, measured the synthesis of collagen type III; Pro-C6, measured the synthesis of collagen type VI; and C6M measured the degradation of collagen type VI induced by MMP-2 and MMP-9 cleavage. RESULTS: Pro-C3 and Pro-C6 biomarkers are up-regulated with both immobilization and remobilization, whereas C6M is hardly affected at all. We found that Pro-C3 and C6M levels are related to LBM at baseline and that high levels of Pro-C6 are associated with smaller changes in muscle mass during both immobilization and remobilization. CONCLUSION: The Pro-C3 and-C6 biomarkers change likely reflect remodeling changes in response to unloading or reloading, whereas C6M does not appear to respond to unloading. Pro-C3 and C6M levels correlate with LBM at baseline, while Pro-C6 is related to the anabolic and catabolic responses to unloading and reloading.
Language eng
DOI 10.1371/journal.pone.0144525
Field of Research 110602 Exercise Physiology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Public Library of Science (PLoS)
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080920

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Citation counts: TR Web of Science Citation Count  Cited 11 times in TR Web of Science
Scopus Citation Count Cited 12 times in Scopus
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.