Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma

Albarhani, Ali A., Collier, Fiona, Greaves, Ronda F., Ponsonby, Anne-Louise, Allen, Katrina J., Vuillermin, Peter J., Roche, Peter and Clarke, Michael W. 2015, Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma, Clinical biochemistry, vol. 48, no. 16-17, pp. 1105-1112, doi: 10.1016/j.clinbiochem.2015.04.014.

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Title Vitamins D and A can be successfully measured by LC-MS/MS in cord blood diluted plasma
Author(s) Albarhani, Ali A.
Collier, Fiona
Greaves, Ronda F.
Ponsonby, Anne-Louise
Allen, Katrina J.
Vuillermin, Peter J.
Roche, Peter
Clarke, Michael W.
Journal name Clinical biochemistry
Volume number 48
Issue number 16-17
Start page 1105
End page 1112
Total pages 8
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-11
ISSN 1873-2933
Keyword(s) Cholecalciferol
Mass spectrometry
Umbilical cord blood diluted plasma
BIS Steering Committee
Science & Technology
Life Sciences & Biomedicine
Medical Laboratory Technology
Summary OBJECTIVES: In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for vitamin D, A and E analysis compared to UCB serum samples. DESIGN & METHODS: Twenty UCB matched samples of diluted plasma and serum were collected. The samples were analysed by two liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods on two separate occasions. RESULTS: The results of 25(OH)D3 obtained by the two laboratories demonstrated close agreement with a mean difference of 0.14nmol/L [95% confidence interval (95% CI), -6.8 to 7.1]. Both methods demonstrate close agreement for 25(OH)D3 in UCB serum versus diluted UCB plasma; mean difference 2.2nmol/L [95% CI, -9.5 to 13.9] and 4.1nmol/L [95% CI, -14.5 to 6.1] for the results from Lab A and Lab B, respectively. Vitamin A was quantified by Lab A in UCB serum and diluted UCB plasma; mean difference 0.07μmol/L [95% CI, -0.41 to 0.28]. Results of 25(OH)D3 epimer and vitamin E in the diluted UCB plasma were below the limit of quantification, and could not be compared with UCB serum. CONCLUSIONS: Diluted UCB plasma can be used for the quantification of retinol and 25(OH)D3 by LC-MS/MS. By contrast, quantification of 25(OH)D3 epimer and vitamin E in diluted UCB plasma is not supported by this study due to limitations in analytical sensitivity.
Language eng
DOI 10.1016/j.clinbiochem.2015.04.014
Field of Research 1101 Medical Biochemistry And Metabolomics
111401 Foetal Development and Medicine
Socio Economic Objective 920299 Health and Support Services not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
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Document type: Journal Article
Collection: School of Medicine
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