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Potential degrading effect of sodium hypochlorite on exhibits containing DNA

Szkuta, B., Ballantyne, K.N. and Van Oorschot, R.A.H. 2015, Potential degrading effect of sodium hypochlorite on exhibits containing DNA, Forensic science international: genetics supplement series, vol. 5, pp. 52-54, doi: 10.1016/j.fsigss.2015.09.021.

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Title Potential degrading effect of sodium hypochlorite on exhibits containing DNA
Author(s) Szkuta, B.
Ballantyne, K.N.
Van Oorschot, R.A.H.
Journal name Forensic science international: genetics supplement series
Volume number 5
Start page 52
End page 54
Total pages 3
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-12
ISSN 1875-1768
1875-175X
Summary The use of a sodium hypochlorite solution as a cleaning reagent is common practice among many laboratories for contamination minimisation purposes. Whilst its effectiveness in the decontamination of tools and surfaces has been verified at specific concentrations, it has not yet been established whether any residual sodium hypochlorite potentially remaining on tools/surfaces following cleaning has a detrimental effect if direct contact is made with an exhibit containing DNA. To investigate the effect of residual hypochlorite, surfaces were treated with 10% hypochlorite (air-dried or wiped dry), 1% hypochlorite (air-dried or wiped dry), or 1% hypochlorite (wiped dry) followed by the application of water (wiped dry). Treated surfaces came into contact with surfaces carrying 200. ng of DNA within 100. μL, or 20. ng within 20. μL. To observe the potential degrading effects of sodium hypochlorite, the quantity and quality of DNA within DNA deposits following contact with treated and untreated surfaces were compared. Overall, no degrading effect on DNA quantity/quality was observed, with the exception of DNA deposits that came into contact with surfaces treated with 10% hypochlorite and air-dried. It is therefore recommended that surfaces cleaned with high concentrations of hypochlorite be wiped dry or rinsed with an appropriate agent (water) following application.
Language eng
DOI 10.1016/j.fsigss.2015.09.021
Field of Research 069901 Forensic Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30081020

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