Heterogeneity of miR-10b expression in circulating tumor cells

Gasch, Christin, Plummer, Prue N., Jovanovic, Lidija, McInnes, Linda M., Wescott, David, Saunders, Christobel M., Schneeweiss, Andreas, Wallwiener, Markus, Nelson, Colleen, Spring, Kevin J., Riethdorf, Sabine, Thompson, Erik W., Pantel, Klaus and Mellick, Albert S. 2015, Heterogeneity of miR-10b expression in circulating tumor cells, Scientific reports, vol. 5, Article Number : 15980, pp. 1-10, doi: 10.1038/srep15980.

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Title Heterogeneity of miR-10b expression in circulating tumor cells
Author(s) Gasch, Christin
Plummer, Prue N.
Jovanovic, Lidija
McInnes, Linda M.
Wescott, David
Saunders, Christobel M.
Schneeweiss, Andreas
Wallwiener, Markus
Nelson, Colleen
Spring, Kevin J.
Riethdorf, Sabine
Thompson, Erik W.
Pantel, Klaus
Mellick, Albert S.
Journal name Scientific reports
Volume number 5
Season Article Number : 15980
Start page 1
End page 10
Total pages 10
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2015
ISSN 2045-2322
Summary Circulating tumor cells (CTCs) in the blood of cancer patients are recognized as important potential targets for future anticancer therapies. As mediators of metastatic spread, CTCs are also promising to be used as € liquid biopsyto aid clinical decision-making. Recent work has revealed potentially important genotypic and phenotypic heterogeneity within CTC populations, even within the same patient. MicroRNAs (miRNAs) are key regulators of gene expression and have emerged as potentially important diagnostic markers and targets for anti-cancer therapy. Here, we describe a robust in situ hybridization (ISH) protocol, incorporating the CellSearch ® CTC detection system, enabling clinical investigation of important miRNAs, such as miR-10b on a cell by cell basis. We also use this method to demonstrate heterogeneity of such as miR-10b on a cell-by-cell basis. We also use this method to demonstrate heterogeneity of miR-10b in individual CTCs from breast, prostate and colorectal cancer patients.
Language eng
DOI 10.1038/srep15980
Field of Research 111299 Oncology and Carcinogenesis not elsewhere classified
Socio Economic Objective 920499 Public Health (excl. Specific Population Health) not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Nature Publishing Group
Persistent URL http://hdl.handle.net/10536/DRO/DU:30081067

Document type: Journal Article
Collection: School of Medicine
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