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PKR is not obligatory for high-fat diet-induced obesity and its associated metabolic and inflammatory complications

Lancaster, G. I., Kammoun, H. L., Kraakman, M. J., Kowalski, G. M., Bruce, C. and Febbraio, M. A. 2016, PKR is not obligatory for high-fat diet-induced obesity and its associated metabolic and inflammatory complications, Nature communications, vol. 7, pp. 1-10, doi: 10.1038/ncomms10626.

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Title PKR is not obligatory for high-fat diet-induced obesity and its associated metabolic and inflammatory complications
Author(s) Lancaster, G. I.
Kammoun, H. L.
Kraakman, M. J.
Kowalski, G. M.ORCID iD for Kowalski, G. M. orcid.org/0000-0002-1599-017X
Bruce, C.ORCID iD for Bruce, C. orcid.org/0000-0002-0515-3343
Febbraio, M. A.
Journal name Nature communications
Volume number 7
Start page 1
End page 10
Total pages 10
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2016-02-03
ISSN 2041-1723
Summary Protein kinase R (PKR) has previously been suggested to mediate many of the deleterious consequences of a high-fat diet (HFD). However, previous studies have observed substantial phenotypic variability when examining the metabolic consequences of PKR deletion. Accordingly, herein, we have re-examined the role of PKR in the development of obesity and its associated metabolic complications in vivo as well as its putative lipid-sensing role in vitro. Here we show that the deletion of PKR does not affect HFD-induced obesity, hepatic steatosis or glucose metabolism, and only modestly affects adipose tissue inflammation. Treatment with the saturated fatty acid palmitate in vitro induced comparable levels of inflammation in WT and PKR KO macrophages, demonstrating that PKR is not necessary for the sensing of pro-inflammatory lipids. These results challenge the proposed role for PKR in obesity, its associated metabolic complications and its role in lipid-induced inflammation.
Language eng
DOI 10.1038/ncomms10626
Field of Research 060104 Cell Metabolism
060603 Animal Physiology - Systems
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30081179

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.