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Osteoclast formation elicited by interleukin-33 stimulation is dependent upon the type of osteoclast progenitor

Eeles, Damien G., Hodge, Jason, Singh, Preetinder P., Schuijers, Johannes A., Grills, Brian L., Gillespie, Matthew T., Myers, Damian E. and Quinn, Julian M. W. 2015, Osteoclast formation elicited by interleukin-33 stimulation is dependent upon the type of osteoclast progenitor, Molecular and cellular endocrinology, vol. 399, pp. 259-266, doi: 10.1016/j.mce.2014.10.014.

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Title Osteoclast formation elicited by interleukin-33 stimulation is dependent upon the type of osteoclast progenitor
Author(s) Eeles, Damien G.
Hodge, Jason
Singh, Preetinder P.
Schuijers, Johannes A.
Grills, Brian L.
Gillespie, Matthew T.
Myers, Damian E.
Quinn, Julian M. W.
Journal name Molecular and cellular endocrinology
Volume number 399
Start page 259
End page 266
Total pages 8
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-01-05
ISSN 1872-8057
1872-8057
Keyword(s) bone resorption
interleukin
osteoclast
Th2 cytokine
Summary Osteoclasts are bone resorbing multinucleated cells (MNCs) derived from macrophage progenitors. IL-33 has been reported to drive osteoclastogenesis independently of receptor activator of NFκB ligand (RANKL) but this remains controversial as later studies did not confirm this. We found IL-33 clearly elicited functional dentine-resorbing osteoclast formation from human adult monocytes. However, monocytes from only 3 of 12 donors responded this way, while all responded to RANKL. Human cord blood-derived progenitors and murine bone marrow macrophages lacked an osteoclastogenic response to IL-33. In RAW264.7 cells, IL-33 elicited NFκB and p38 responses but not NFATc1 signals (suggesting poor osteoclastogenic responses) and formed only mononuclear tartrate-resistant acid phosphatase positive (TRAP(+)) cells. Since TGFβ boosts osteoclastogenesis in RAW264.7 cells we employed an IL-33/TGFβ co-treatment, which resulted in small numbers of MNCs expressing key osteoclast markers TRAP and calcitonin receptors. Thus, IL-33 possesses weak osteoclastogenic activity suggesting pathological significance and, perhaps, explaining previous conflicting reports.
Language eng
DOI 10.1016/j.mce.2014.10.014
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30081331

Document type: Journal Article
Collection: School of Medicine
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