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Evaluation of nanoformulated therapeutics in an ex-vivo bovine corneal irritation model

Bhasker, Sriramoju, Kislay, Roy, Rupinder, Kanwar K. and Jagat, Kanwar R. 2015, Evaluation of nanoformulated therapeutics in an ex-vivo bovine corneal irritation model, Toxicology in vitro, vol. 29, no. 5, pp. 917-925, doi: 10.1016/j.tiv.2015.01.007.

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Title Evaluation of nanoformulated therapeutics in an ex-vivo bovine corneal irritation model
Author(s) Bhasker, Sriramoju
Kislay, Roy
Rupinder, Kanwar K.
Jagat, Kanwar R.ORCID iD for Jagat, Kanwar R. orcid.org/0000-0003-3728-9568
Journal name Toxicology in vitro
Volume number 29
Issue number 5
Start page 917
End page 925
Total pages 9
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-08
ISSN 0887-2333
Keyword(s) Corneal irritation
PLGA
Chitosan
SurR9-C84A
Bovine lactoferrin
Summary Aim : To determine the internalization and protective effects of potential ophthalmic formulations and nanoformulated natural proteins in ex-vivo bovine corneal alkali burn model.

Methods : The bovine cornea obtained were subjected to the 0.5 N NaOH insult that induced alkali burn and inflammation as observed in the in vivo situation. The toxic effects of the nanoformulation were evaluated in the normal and insult induced cornea using histological analysis. Internalization studies were carried out using in vivo imaging and analysis (IVIS, PerkinElmer, USA).

Results : The nanoformulations employed in this study showed no obvious changes in the integrity of the cornea. Further, improvements in the light transmittance and reduced inflammation were observed. The IVIS showed a dose dependant increase in the uptake of the nanoformulations with time.

Conclusion : The nanoformulated bovine lactoferrin and SurR9-C84A (SR9) proteins evaluated in the ex vivo bovine corneal irritation model is the first of its kind, and we report here the non-toxic and therapeutic potential of these formulations for topical applications.
Language eng
DOI 10.1016/j.tiv.2015.01.007
Field of Research 1115 Pharmacology And Pharmaceutical Sciences
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30081475

Document type: Journal Article
Collection: School of Medicine
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