Clinical MRSA isolates from skin and soft tissue infections show increased in vitro production of phenol soluble modulins

Berlon, Nicholas R., Qi, Robert, Sharma-Kuinkel, Batu K., Joo, Hwang-Soo, Park, Lawrence P., George, Dennis, Thaden, Joshua T., Messina, Julia A., Maskarinec, Stacey A., Mueller-Premru, Manica, Athan, Eugene, Tattevin, Pierre, Pericas, Juan M., Woods, Christopher W., Otto, Michael and Fowler, Vance G. 2015, Clinical MRSA isolates from skin and soft tissue infections show increased in vitro production of phenol soluble modulins, Journal of infection, vol. 71, no. 4, pp. 447-457, doi: 10.1016/j.jinf.2015.06.005.

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Title Clinical MRSA isolates from skin and soft tissue infections show increased in vitro production of phenol soluble modulins
Author(s) Berlon, Nicholas R.
Qi, Robert
Sharma-Kuinkel, Batu K.
Joo, Hwang-Soo
Park, Lawrence P.
George, Dennis
Thaden, Joshua T.
Messina, Julia A.
Maskarinec, Stacey A.
Mueller-Premru, Manica
Athan, EugeneORCID iD for Athan, Eugene orcid.org/0000-0001-9838-6471
Tattevin, Pierre
Pericas, Juan M.
Woods, Christopher W.
Otto, Michael
Fowler, Vance G.
Journal name Journal of infection
Volume number 71
Issue number 4
Start page 447
End page 457
Total pages 11
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-10
ISSN 1532-2742
Keyword(s) Endocarditis
MRSA
Phenol soluble modulin
Pneumonia
Skin and soft tissue infection
Science & Technology
Life Sciences & Biomedicine
Infectious Diseases
Skin and soft infection
RESISTANT STAPHYLOCOCCUS-AUREUS
COAGULASE-NEGATIVE STAPHYLOCOCCI
GENE REGULATOR AGR
UNITED-STATES
VIRULENCE DETERMINANTS
COMPLICATED SKIN
PCR ASSAY
METHICILLIN
VANCOMYCIN
Summary BACKGROUND: Phenol-soluble modulins (PSMs) are amphipathic, pro-inflammatory proteins secreted by most Staphylococcus aureus isolates. This study tested the hypothesis that in vitro PSM production levels are associated with specific clinical phenotypes. METHODS: 177 methicillin-resistant S. aureus (MRSA) isolates from infective endocarditis (IE), skin and soft tissue infection (SSTI), and hospital-acquired/ventilator-associated pneumonia (HAP) were matched by geographic origin, then genotyped using spa-typing. In vitro PSM production was measured by high performance liquid chromatography/mass spectrometry. Statistical analysis was performed using Chi-squared or Kruskal-Wallis tests as appropriate. RESULTS: Spa type 1 was significantly more common in SSTI isolates (62.7% SSTI; 1.7% IE; 16.9% HAP; p < 0.0001) while HAP and IE isolates were more commonly spa type 2 (0% SSTI; 37.3% IE; 40.7% HAP; p < 0.0001). USA300 isolates produced the highest levels of PSMs in vitro. SSTI isolates produced significantly higher quantities of PSMα1-4, PSMβ1, and δ-toxin than other isolates (p < 0.001). These findings persisted when USA300 isolates were excluded from analysis. CONCLUSIONS: Increased in vitro production of PSMs is associated with an SSTI clinical source. This significant association persisted after exclusion of USA300 genotype isolates from analysis, suggesting that PSMs play a particularly important role in the pathogenesis of SSTI as compared to other infection types.
Language eng
DOI 10.1016/j.jinf.2015.06.005
Field of Research 1103 Clinical Sciences
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30081598

Document type: Journal Article
Collection: School of Medicine
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