Bioprocess challenges to the isolation and purification of bioactive peptides

Agyei, Dominic, Ongkudon, Clarence M, Wei, Chan Yi, Chan, Alan S and Danquah, Michael K 2016, Bioprocess challenges to the isolation and purification of bioactive peptides, Food and bioproducts processing, vol. 96, pp. 244-256, doi: 10.1016/j.fbp.2016.02.003.

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Title Bioprocess challenges to the isolation and purification of bioactive peptides
Author(s) Agyei, DominicORCID iD for Agyei, Dominic orcid.org/0000-0003-2280-4096
Ongkudon, Clarence M
Wei, Chan Yi
Chan, Alan S
Danquah, Michael K
Journal name Food and bioproducts processing
Volume number 96
Start page 244
End page 256
Total pages 13
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-04
ISSN 1744-3571
Keyword(s) Bioactive peptides
Functional foods
Bioprocessing
Peptidomics
In silico analysis
Monolithic chromatography
Summary Food protein-derived bioactive peptides (BPs) have been reported to trigger certain physiological responses in the body, thereby influencing health positively. These peptides have attracted high research and consumer interests due to their huge potential of use in functional foods and other dietary interventions of disease control and health promotion. However, successful product development is limited by the fact that current manufacturing processes are either difficult to scale up, high in cost, or have the potential to affect the structure-activity properties of these peptides. To overcome these challenges, we have proposed in this review, the use of an integrated ‘-omics’ approach comprising in silico analysis and ‘-omics’ techniques (such as peptidomics) to respectively forecast and validate the biological and physico-chemical properties of the peptides. This information is then used for the rational design of suitable purification steps for peptides of interest. Downstream purification could also be undertaken by liquid chromatography using monolithic adsorbents physico-chemically engineered (using results of in silico analysis) for rapid isolation of peptides. By coupling the high throughput and predictive capability of ‘-omics’ to the enhanced convective hydrodynamics of monolithic columns, it becomes feasible, even at preparative scale, to produce BPs that meet the requirements of high purity, potency, and cost-effectiveness.
Language eng
DOI 10.1016/j.fbp.2016.02.003
Field of Research 090899 Food Sciences not elsewhere classified
119999 Medical and Health Sciences not elsewhere classified
100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 860899 Human Pharmaceutical Products not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30081807

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