Sequence type 131 fimH30 and fimH41 subclones amongst escherichia coli isolates in Australia and New Zealand

Rogers, Benjamin A., Ingram, Paul R., Runnegar, Naomi, Pitman, Matthew C., Freeman, Joshua T., Athan, Eugene, Havers, Sally, Sidjabat, Hanna E., Gunning, Earleen, De Almeida, Mary, Styles, Kaylene and Paterson, David L. 2015, Sequence type 131 fimH30 and fimH41 subclones amongst escherichia coli isolates in Australia and New Zealand, International journal of antimicrobial agents, vol. 45, no. 4, pp. 351-358, doi: 10.1016/j.ijantimicag.2014.11.015.

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Title Sequence type 131 fimH30 and fimH41 subclones amongst escherichia coli isolates in Australia and New Zealand
Author(s) Rogers, Benjamin A.
Ingram, Paul R.
Runnegar, Naomi
Pitman, Matthew C.
Freeman, Joshua T.
Athan, EugeneORCID iD for Athan, Eugene
Havers, Sally
Sidjabat, Hanna E.
Gunning, Earleen
De Almeida, Mary
Styles, Kaylene
Paterson, David L.
Journal name International journal of antimicrobial agents
Volume number 45
Issue number 4
Start page 351
End page 358
Total pages 8
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-04
ISSN 1872-7913
Keyword(s) Cephalosporin resistance
Escherichia coli
Urinary tract infection
Science & Technology
Life Sciences & Biomedicine
Infectious Diseases
Pharmacology & Pharmacy
Summary The clonal composition of Escherichia coli causing extra-intestinal infections includes ST131 and other common uropathogenic clones. Drivers for the spread of these clones and risks for their acquisition have been difficult to define. In this study, molecular epidemiology was combined with clinical data from 182 patients enrolled in a case-control study of community-onset expanded-spectrum cephalosporin-resistant E. coli (ESC-R-EC) in Australia and New Zealand. Genetic analysis included antimicrobial resistance mechanisms, clonality by DiversiLab (rep-PCR) and multilocus sequence typing (MLST), and subtyping of ST131 by identification of polymorphisms in the fimH gene. The clonal composition of expanded-spectrum cephalosporin-susceptible E. coli and ESC-R-EC isolates differed, with six MLST clusters amongst susceptible isolates (median 7 isolates/cluster) and three clusters amongst resistant isolates, including 40 (45%) ST131 isolates. Population estimates indicate that ST131 comprises 8% of all E. coli within our population; the fluoroquinolone-susceptible H41 subclone comprised 4.5% and the H30 subclone comprised 3.5%. The H30 subclone comprised 39% of all ESC-R-EC and 41% of all fluoroquinolone-resistant E. coli within our population. Patients with ST131 were also more likely than those with non-ST131 isolates to present with an upper than lower urinary tract infection (RR=1.8, 95% CI 1.01-3.1). ST131 and the H30 subclone were predominant amongst ESC-R-EC but were infrequent amongst susceptible isolates where the H41 subclone was more prevalent. Within our population, the proportional contribution of ST131 to fluoroquinolone resistance is comparable with that of other regions. In contrast, the overall burden of ST131 is low by global standards.
Language eng
DOI 10.1016/j.ijantimicag.2014.11.015
Field of Research 1108 Medical Microbiology
1115 Pharmacology And Pharmaceutical Sciences
110899 Medical Microbiology not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
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