Pan-serotype diagnostic for foot-and-mouth disease using the consensus antigen of nonstructural protein 3B

Van Dreumel, Alyssa K., Michalski, Wojtek P., McNabb, Leanne M., Shiell, Brian J., Singanallur, Nagendrakumar B. and Peck, Grantley R. 2015, Pan-serotype diagnostic for foot-and-mouth disease using the consensus antigen of nonstructural protein 3B, Journal of clinical microbiology, vol. 53, no. 6, pp. 1797-1805, doi: 10.1128/JCM.03491-14.

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Title Pan-serotype diagnostic for foot-and-mouth disease using the consensus antigen of nonstructural protein 3B
Author(s) Van Dreumel, Alyssa K.
Michalski, Wojtek P.
McNabb, Leanne M.
Shiell, Brian J.
Singanallur, Nagendrakumar B.
Peck, Grantley R.
Contributor(s) Loeffelholz, M. J.
Journal name Journal of clinical microbiology
Volume number 53
Issue number 6
Start page 1797
End page 1805
Total pages 9
Publisher American Society for Microbiology
Place of publication Washington, DC
Publication date 2015-06
ISSN 0095-1137
Summary An amino acid consensus sequence for the seven serotypes of foot-and-mouth disease virus (FMDV) nonstructural protein 3B, including all three contiguous repeats, and its use in the development of a pan-serotype diagnostic test for all seven FMDV serotypes are described. The amino acid consensus sequence of the 3B protein was determined from a multiple-sequence alignment of 125 sequences of 3B. The consensus 3B (c3B) protein was expressed as a soluble recombinant fusion protein with maltose-binding protein (MBP) using a bacterial expression system and was affinity purified using amylose resin. The MBP-c3B protein was used as the antigen in the development of a competition enzyme-linked immunosorbent assay (cELISA) for detection of anti-3B antibodies in bovine sera. The comparative diagnostic sensitivity and specificity at 47% inhibition were estimated to be 87.22% and 93.15%, respectively. Reactivity of c3B with bovine sera representing the seven FMDV serotypes demonstrated the pan-serotype diagnostic capability of this bioreagent. The consensus antigen and competition ELISA are described here as candidates for a pan-serotype diagnostic test for FMDV infection.
Language eng
DOI 10.1128/JCM.03491-14
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, American Society for Microbiology
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