Pan-serotype diagnostic for foot-and-mouth disease using the consensus antigen of nonstructural protein 3B Van Dreumel, Alyssa K., Michalski, Wojtek P., McNabb, Leanne M., Shiell, Brian J., Singanallur, Nagendrakumar B. and Peck, Grantley R. 2015, Pan-serotype diagnostic for foot-and-mouth disease using the consensus antigen of nonstructural protein 3B, Journal of clinical microbiology, vol. 53, no. 6, pp. 1797-1805, doi: 10.1128/JCM.03491-14. Attached Files Name Description MIMEType Size Downloads Title Pan-serotype diagnostic for foot-and-mouth disease using the consensus antigen of nonstructural protein 3B Author(s) Van Dreumel, Alyssa K. Michalski, Wojtek P. McNabb, Leanne M. Shiell, Brian J. Singanallur, Nagendrakumar B. Peck, Grantley R. Contributor(s) Loeffelholz, M. J. Journal name Journal of clinical microbiology Volume number 53 Issue number 6 Start page 1797 End page 1805 Total pages 9 Publisher American Society for Microbiology Place of publication Washington, DC Publication date 2015-06 ISSN 0095-1137 1098-660X Summary An amino acid consensus sequence for the seven serotypes of foot-and-mouth disease virus (FMDV) nonstructural protein 3B, including all three contiguous repeats, and its use in the development of a pan-serotype diagnostic test for all seven FMDV serotypes are described. The amino acid consensus sequence of the 3B protein was determined from a multiple-sequence alignment of 125 sequences of 3B. The consensus 3B (c3B) protein was expressed as a soluble recombinant fusion protein with maltose-binding protein (MBP) using a bacterial expression system and was affinity purified using amylose resin. The MBP-c3B protein was used as the antigen in the development of a competition enzyme-linked immunosorbent assay (cELISA) for detection of anti-3B antibodies in bovine sera. The comparative diagnostic sensitivity and specificity at 47% inhibition were estimated to be 87.22% and 93.15%, respectively. Reactivity of c3B with bovine sera representing the seven FMDV serotypes demonstrated the pan-serotype diagnostic capability of this bioreagent. The consensus antigen and competition ELISA are described here as candidates for a pan-serotype diagnostic test for FMDV infection. Language eng DOI 10.1128/JCM.03491-14 Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 929999 Health not elsewhere classified HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2015, American Society for Microbiology Persistent URL http://hdl.handle.net/10536/DRO/DU:30082088 Document type: Journal Article Collection: School of Medicine Related Links Link Description Connect to Elements publication management system Go to link with your DU access privileges   Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Thu, 10 Mar 2016, 14:26:44 EST Thu, 10 Mar 2016, 14:39:28 EST Thu, 10 Mar 2016, 15:00:26 EST Tue, 05 Jul 2016, 11:42:37 EST Tue, 05 Jul 2016, 11:50:50 EST Tue, 19 Jul 2016, 09:45:29 EST Wed, 12 Oct 2016, 14:56:57 EST Wed, 01 Mar 2017, 07:50:32 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 41 Abstract Views, 2 File Downloads  -  Detailed Statistics Created: Thu, 10 Mar 2016, 14:26:44 EST

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