Metabolites derived from the tropical seagrass Thalassia testudinum are bioactive against pathogenic Labyrinthula sp

Trevathan-Tackett, Stacey M., Lane, Amy L., Bishop, Nichole and Ross, Cliff 2015, Metabolites derived from the tropical seagrass Thalassia testudinum are bioactive against pathogenic Labyrinthula sp, Aquatic botany, vol. 122, pp. 1-8, doi: 10.1016/j.aquabot.2014.12.005.

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Title Metabolites derived from the tropical seagrass Thalassia testudinum are bioactive against pathogenic Labyrinthula sp
Author(s) Trevathan-Tackett, Stacey M.ORCID iD for Trevathan-Tackett, Stacey M.
Lane, Amy L.
Bishop, Nichole
Ross, Cliff
Journal name Aquatic botany
Volume number 122
Start page 1
End page 8
Total pages 8
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015
ISSN 0304-3770
Keyword(s) Seagrass defense
Thalassia testudinum
Secondary metabolites
Wasting disease
Summary Temperate and tropical seagrasses are susceptible to wasting disease outbreaks caused by pathogenic protists of the genus Labyrinthula. Even though there is an increasing awareness of the environmental conditions that influence the etiology of seagrass-. Labyrinthula disease dynamics, the biochemical basis of seagrass defense responses, in particular chemical defenses, is still vastly understudied. Using an in vitro bioassay, we provide evidence that previously characterized phenolic and potentially novel, undescribed non-phenolic metabolites derived from Thalassia testudinum Banks ex Konig exhibit anti-labyrinthulid activity. All phenolic compounds tested displayed dose-dependent behavior and selected combinations interacted synergistically. The flavone glycoside thalassiolin B was roughly 20-100 times more active than any phenolic acid tested. Based upon values reported in the literature, it was calculated that infected specimens of T. testudinum contain natural concentrations of phenolic acids that are consistently greater than what is required to inhibit Labyrinthula growth. This suggests that while there may be an ample supply of phenolic-based derivatives available to inhibit Labyrinthula growth, they may not be readily bio-accessible.Using a bioactivity-guided approach, a semi-purified chemical fraction from T. testudinum was found to contain anti-labyrinthulid activity. 1H NMR spectra for this fraction lacked aromatic hydrogen signals, suggesting that the bioactive compound was non-aromatic in nature. Furthermore, the LC-MS fragmentation patterns were suggestive of the presence of glycosylated natural products of an unknown structural class. This has the potential to provide a foundation for future chemical investigations.
Language eng
DOI 10.1016/j.aquabot.2014.12.005
Field of Research 060205 Marine and Estuarine Ecology (incl Marine Ichthyology)
060701 Phycology (incl Marine Grasses)
050205 Environmental Management
0607 Plant Biology
0502 Environmental Science And Management
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
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