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Microfluidic device to measure the speed of C. elegans using the resistance change of the flexible electrode

Jung, Jaehoon, Nakajima, Masahiro, Takeuchi, Masaru, Najdovski, Zoran, Huang, Qiang and Fukuda, Toshio 2016, Microfluidic device to measure the speed of C. elegans using the resistance change of the flexible electrode, Micromachines, vol. 7, no. 3, pp. 1-12, doi: 10.3390/mi7030050.

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Title Microfluidic device to measure the speed of C. elegans using the resistance change of the flexible electrode
Formatted title  Microfluidic device to measure the speed of C. elegans using the resistance change of the flexible electrode
Author(s) Jung, Jaehoon
Nakajima, Masahiro
Takeuchi, Masaru
Najdovski, ZoranORCID iD for Najdovski, Zoran orcid.org/0000-0002-8880-8287
Huang, Qiang
Fukuda, Toshio
Journal name Micromachines
Volume number 7
Issue number 3
Start page 1
End page 12
Total pages 12
Publisher MDPI AG
Place of publication Basel, Switzerland
Publication date 2016-03-19
ISSN 2072-666X
Keyword(s) microfluidic device
C. elegans
flexible electrode
Science & Technology
Technology
Nanoscience & Nanotechnology
Instruments & Instrumentation
Science & Technology - Other Topics
NEMATODE CAENORHABDITIS-ELEGANS
WILD-TYPE
LOCOMOTION
TOXICITY
MODEL
EXPOSURE
BEHAVIOR
DISEASES
SYSTEM
RNAI
Summary This work presents a novel method to assess the condition of Caenorhabditis elegans (C. elegans) through a resistance measurement of its undulatory locomotion speed inside a micro channel. As the worm moves over the electrode inside the micro channel, the length of the electrode changes, consequently behaving like a strain gauge. In this paper, the electrotaxis was applied for controlling the direction of motion of C. elegans as an external stimulus, resulting in the worm moving towards the cathode of the circuit. To confirm the proposed measurement method, a microfluidic device was developed that employs a sinusoidal channel and a thin polydimethylsiloxane (PDMS) layer with an electrode. The PDMS layer maintains a porous structure to enable the flexibility of the electrode. In this study, 6 measurements were performed to obtain the speed of an early adult stage C. elegans, where the measured average speed was 0.35 (±0.05) mm/s. The results of this work demonstrate the application of our method to measure the speed of C. elegans undulatory locomotion. This novel approach can be applied to make such measurements without an imaging system, and more importantly, allows directly to detect the locomotion of C. elegans using an electrical signal (i.e., the change in resistance).
Language eng
DOI 10.3390/mi7030050
Field of Research 099999 Engineering not elsewhere classified
Socio Economic Objective 0 Not Applicable
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30082395

Document type: Journal Article
Collections: Centre for Intelligent Systems Research
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.