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Isolation and functional Characterisation of a fads2 in Rainbow Trout (Oncorhynchus mykiss) with Δ5 desaturase activity

Abdul Hamid, Noor Khalidah, Carmona-Antoñanzas, Greta, Monroig, Óscar, Tocher, Douglas R., Turchini, Giovanni M. and Donald, John A. 2016, Isolation and functional Characterisation of a fads2 in Rainbow Trout (Oncorhynchus mykiss) with Δ5 desaturase activity, PLoS one, vol. 11, no. 3, pp. 1-16, doi: 10.1371/journal.pone.0150770.

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Title Isolation and functional Characterisation of a fads2 in Rainbow Trout (Oncorhynchus mykiss) with Δ5 desaturase activity
Formatted title Isolation and functional Characterisation of a fads2 in Rainbow Trout (Oncorhynchus mykiss) with Δ5 desaturase activity
Author(s) Abdul Hamid, Noor Khalidah
Carmona-Antoñanzas, Greta
Monroig, Óscar
Tocher, Douglas R.
Turchini, Giovanni M.ORCID iD for Turchini, Giovanni M. orcid.org/0000-0003-0694-4283
Donald, John A.ORCID iD for Donald, John A. orcid.org/0000-0001-5930-2642
Journal name PLoS one
Volume number 11
Issue number 3
Start page 1
End page 16
Total pages 16
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2016-03-04
ISSN 1932-6203
Summary Rainbow trout, Oncorhynchus mykiss, are intensively cultured globally. Understanding their requirement for long-chain polyunsaturated fatty acids (LC-PUFA) and the biochemistry of the enzymes and biosynthetic pathways required for fatty acid synthesis is important and highly relevant in current aquaculture. Most gnathostome vertebrates have two fatty acid desaturase (fads) genes with known functions in LC-PUFA biosynthesis and termed fads1 and fads2. However, teleost fish have exclusively fads2 genes. In rainbow trout, a fads2 cDNA had been previously cloned and found to encode an enzyme with Δ6 desaturase activity. In the present study, a second fads2 cDNA was cloned from the liver of rainbow trout and termed fads2b. The full-length mRNA contained 1578 nucleotides with an open reading frame of 1365 nucleotides that encoded a 454 amino acid protein with a predicted molecular weight of 52.48 kDa. The predicted Fads2b protein had the characteristic traits of the microsomal Fads family, including an N-terminal cytochrome b5 domain containing the heme-binding motif (HPPG), histidine boxes (HDXGH, HFQHH and QIEHH) and three transmembrane regions. The fads2b was expressed predominantly in the brain, liver, intestine and pyloric caeca. Expression of the fasd2b in yeast generated a protein that was found to specifically convert eicosatetraenoic acid (20:4n-3) to eicosapentaenoic acid (20:5n-3), and therefore functioned as a Δ5 desaturase. Therefore, rainbow trout have two fads2 genes that encode proteins with Δ5 and Δ6 desaturase activities, respectively, which enable this species to perform all the desaturation steps required for the biosynthesis of LC-PUFA from C18 precursors.
Language eng
DOI 10.1371/journal.pone.0150770
Field of Research 070401 Aquaculture
Socio Economic Objective 830102 Aquaculture Fin Fish (excl. Tuna)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30082473

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Created: Wed, 30 Mar 2016, 11:25:36 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.