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Overcoming solvent mismatch limitations in 2D-HPLC with temperature programming of isocratic mobile phases

Holland, Brendan J., Conlan, Xavier A., Francis, Paul S., Barnett, Neil W. and Stevenson, Paul G. 2016, Overcoming solvent mismatch limitations in 2D-HPLC with temperature programming of isocratic mobile phases, Analytical methods, vol. 8, no. 6, pp. 1293-1298, doi: 10.1039/c5ay02528a.

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Title Overcoming solvent mismatch limitations in 2D-HPLC with temperature programming of isocratic mobile phases
Author(s) Holland, Brendan J.
Conlan, Xavier A.ORCID iD for Conlan, Xavier A. orcid.org/0000-0003-0829-0551
Francis, Paul S.ORCID iD for Francis, Paul S. orcid.org/0000-0003-4165-6922
Barnett, Neil W.
Stevenson, Paul G.ORCID iD for Stevenson, Paul G. orcid.org/0000-0001-6780-6859
Journal name Analytical methods
Volume number 8
Issue number 6
Start page 1293
End page 1298
Total pages 6
Publisher Royal Society of Chemistry
Place of publication Cambridge, Eng.
Publication date 2016
ISSN 1759-9660
1759-9679
Keyword(s) Temperature programming
Solvent mismatch limitations
Science & Technology
Physical Sciences
Life Sciences & Biomedicine
Technology
Chemistry, Analytical
Food Science & Technology
Spectroscopy
Chemistry
2-DIMENSIONAL LIQUID-CHROMATOGRAPHY
GRADIENT ELUTION
PEAK-CAPACITY
PERFORMANCE
SEPARATION
ORTHOGONALITY
INJECTION
2ND-DIMENSION
DIMENSIONS
MODE
Summary This work describes a method for two-dimensional high performance liquid chromatography (2D-HPLC) that uses an isocratic mobile phase with a temperature gradient in the first dimension. Temperature programming was used to manipulate solvent elution strength in place of a mobile phase concentration gradient. This ensured that all eluent fractions transferred into the second dimension were of an identical solvent composition, i.e. the second dimension injection solvent did not increase during the course of the analysis. When applied to a complex natural product extract of coffee, the separation was completed in 35 min and had an orthogonality of 35% (calculated using the bins method) and a spreading angle of 52° as determined via a geometric approach to factor analysis. This approach, incorporating a temperature gradient in the first dimension, compared favourably to previously reported 2D-HPLC separations of coffee, with similar or shorter analysis times.
Language eng
DOI 10.1039/c5ay02528a
Field of Research 030108 Separation Science
0301 Analytical Chemistry
0399 Other Chemical Sciences
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID LP130100681
Copyright notice ©2016, Royal Society of Chemistry
Persistent URL http://hdl.handle.net/10536/DRO/DU:30082477

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