pH-sensitive expression of calcium-sensing receptor (CaSR) in type-B intercalated cells of the cortical collecting ducts (CCD) in mouse kidney

Yasuoka, Yukiko, Sato, Yuichi, Healy, Jillian M., Nonoguchi, Hiroshi and Kawahara, Katsumasa 2014, pH-sensitive expression of calcium-sensing receptor (CaSR) in type-B intercalated cells of the cortical collecting ducts (CCD) in mouse kidney, Clinical and experimental nephrology, vol. 19, no. 5, pp. 771-782, doi: 10.1007/s10157-014-1063-1.

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Title pH-sensitive expression of calcium-sensing receptor (CaSR) in type-B intercalated cells of the cortical collecting ducts (CCD) in mouse kidney
Author(s) Yasuoka, Yukiko
Sato, Yuichi
Healy, Jillian M.
Nonoguchi, Hiroshi
Kawahara, Katsumasa
Journal name Clinical and experimental nephrology
Volume number 19
Issue number 5
Start page 771
End page 782
Total pages 12
Publisher Nihon Jinzo Gakkai
Place of publication Tokyo, Japan
Publication date 2014-12-13
ISSN 1342-1751
1437-7799
Keyword(s) calcium-sensing receptor
neomycin
unirary calcium concentration
acid/base diet
kidney collecting duct intercalated cell
Summary Background The localization and role of the calcium-sensing receptor (CaSR) along the nephron including the collecting ducts is still open to debate. Methods Using the quantitative, highly sensitive in situ hybridization technique and a double-staining immunohistochemistry technique, we investigated the axial distribution and expression of CaSR along the nephron in mice (C57B/6J) treated for 6 days with acid or alkali diets. Results Under control condition, CaSR was specifically localized in the cortical and medullary thick ascending limb of Henle’s loop (CTAL and MTAL), macula densa (MD), distal convoluted tubule (DCT), and CCD (TALs, MD > DCT, CCD). Along the CCD, CaSR was co-localized with an anion exchanger type 4 (AE4), a marker of the basolateral membrane of type-B intercalated cell (IC-B) in mice. On the contrary, CaSR was not detected either in principal cells (PC) or in type-A intercalated cell (IC-A). CaSR expression levels in IC-B significantly (P < 0.005) decreased when mice were fed NH4Cl (acid) diets and increased when animals were given NaHCO3 (alkali) diets. As expected, cell heights of IC-A and IC-B significantly (P < 0.005) increased in the above experimental conditions. Surprisingly, single infusion (ip) of neomycin, an agonist of CaSR, significantly (P < 0.005) increased urinary Ca excretion without further increasing the hourly urine volume and significantly (P < 0.05) decreased urine pH. Conclusion CaSR, cloned from rat kidney, was localized in the basolateral membrane of IC-B and was more expressed during alkali-loading. Its alkali-sensitive expression may promote urinary alkali secretion for body acid–base balance.
Language eng
DOI 10.1007/s10157-014-1063-1
Field of Research 060602 Animal Physiology - Cell
Socio Economic Objective 920119 Urogenital System and Disorders
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Japanese Society of Nephrology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30082612

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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