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A quick colorimetric method for total lipid quantification in microalgae

Byreddy, Avinesh R., Gupta, Adarsha, Barrow, Colin J. and Puri, Munish 2016, A quick colorimetric method for total lipid quantification in microalgae, Journal of microbiological methods, vol. 125, pp. 28-32, doi: 10.1016/j.mimet.2016.04.002.

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Title A quick colorimetric method for total lipid quantification in microalgae
Author(s) Byreddy, Avinesh R.
Gupta, Adarsha
Barrow, Colin J.ORCID iD for Barrow, Colin J. orcid.org/0000-0002-2153-7267
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Journal name Journal of microbiological methods
Volume number 125
Start page 28
End page 32
Total pages 5
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-06
ISSN 1872-8359
Keyword(s) algae
biodiesel
esterification
industrial biotechnology
vanillin
Summary Discovering microalgae with high lipid productivity are among the key milestones for achieving sustainable biodiesel production. Current methods of lipid quantification are time intensive and costly. A rapid colorimetric method based on sulpho-phospho-vanillin (SPV) reaction was developed for the quantification of microbial lipids to facilitate screening for lipid producing microalgae. This method was successfully tested on marine thraustochytrid strains and vegetable oils. The colorimetric method results correlated well with gravimetric method estimates. The new method was less time consuming than gravimetric analysis and is quantitative for lipid determination, even in the presence of carbohydrates, proteins and glycerol.
Language eng
DOI 10.1016/j.mimet.2016.04.002
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
1108 Medical Microbiology
Socio Economic Objective 850501 Biofuel (Biomass) Energy
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30082630

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Created: Mon, 18 Apr 2016, 13:34:15 EST

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