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Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss)

Gregory, Melissa K., Collins, Robert O., Tocher, Douglas R., James, Michael J. and Turchini, Giovanni M. 2016, Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss), British journal of nutrition, vol. 115, no. 10, pp. 1721-1729, doi: 10.1017/S0007114516000830.

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Title Nutritional regulation of long-chain PUFA biosynthetic genes in rainbow trout (Oncorhynchus mykiss)
Author(s) Gregory, Melissa K.
Collins, Robert O.
Tocher, Douglas R.
James, Michael J.
Turchini, Giovanni M.ORCID iD for Turchini, Giovanni M. orcid.org/0000-0003-0694-4283
Journal name British journal of nutrition
Volume number 115
Issue number 10
Start page 1721
End page 1729
Total pages 9
Publisher Cambridge University Press
Place of publication Cambridge, Eng.
Publication date 2016-05
ISSN 1475-2662
1475-2662
Keyword(s) n-3 Fatty acids
ALA α-linolenic acid
DPA docosapentaenoic acid
ELOVL elongation of very long-chain fatty acid
FADS fatty acyl desaturase
LC-PUFA long-chain PUFA
DHA
Desaturase
Elongase
PUFA
Summary Most studies on dietary vegetable oil in rainbow trout (Oncorhynchus mykiss) have been conducted on a background of dietary EPA (20 : 5n-3) and DHA (22 : 6n-3) contained in the fishmeal used as a protein source in aquaculture feed. If dietary EPA and DHA repress their endogenous synthesis from α-linolenic acid (ALA, 18 : 3n-3), then the potential of ALA-containing vegetable oils to maintain tissue EPA and DHA has been underestimated. We examined the effect of individual dietary n-3 PUFA on the expression of the biosynthetic genes required for metabolism of ALA to DHA in rainbow trout. A total of 720 juvenile rainbow trout were allocated to twenty-four experimental tanks and assigned one of eight diets. The effect of dietary ALA, EPA or DHA, in isolation or in combination, on hepatic expression of fatty acyl desaturase (FADS)2a(Δ6), FADS2b(Δ5), elongation of very long-chain fatty acid (ELOVL)5 and ELOVL2 was examined after 3 weeks of dietary intervention. The effect of these diets on liver and muscle phospholipid PUFA composition was also examined. The expression levels of FADS2a(Δ6), ELOVL5 and ELOVL2 were highest when diets were high in ALA, with no added EPA or DHA. Under these conditions ALA was readily converted to tissue DHA. Dietary DHA had the largest and most consistent effect in down-regulating the gene expression of all four genes. The ELOVL5 expression was the least responsive of the four genes to dietary n-3 PUFA changes. These findings should be considered when optimising aquaculture feeds containing vegetable oils and/or fish oil or fishmeal to achieve maximum DHA synthesis.
Language eng
DOI 10.1017/S0007114516000830
Field of Research 070401 Aquaculture
0702 Animal Production
1111 Nutrition And Dietetics
0908 Food Sciences
Socio Economic Objective 830102 Aquaculture Fin Fish (excl. Tuna)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Cambridge University Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30082913

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