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Comparative effect of dairy fatty acids on cell adhesion molecules, nitric oxide and relative gene expression in healthy and diabetic human aortic endothelial cells

Livingstone, K.M., Givens, D.I., Jackson, K.G. and Lovegrove, J.A. 2014, Comparative effect of dairy fatty acids on cell adhesion molecules, nitric oxide and relative gene expression in healthy and diabetic human aortic endothelial cells, Atherosclerosis, vol. 234, no. 1, pp. 65-72, doi: 10.1016/j.atherosclerosis.2014.02.015.

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Title Comparative effect of dairy fatty acids on cell adhesion molecules, nitric oxide and relative gene expression in healthy and diabetic human aortic endothelial cells
Author(s) Livingstone, K.M.ORCID iD for Livingstone, K.M. orcid.org/0000-0002-9682-7541
Givens, D.I.
Jackson, K.G.
Lovegrove, J.A.
Journal name Atherosclerosis
Volume number 234
Issue number 1
Start page 65
End page 72
Total pages 8
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2014-05
ISSN 0021-9150
1879-1484
Keyword(s) Cardiovascular disease
Dairy
Endothelial cells
Fatty acids
Type II diabetes
Cell Adhesion Molecules
Cells, Cultured
Dairy Products
Diabetes Mellitus, Type 2
Endothelium, Vascular
Gene Expression
Humans
Nitric Oxide
Summary OBJECTIVE: Dairy intake, despite its high saturated fatty acid (SFA) content, is associated with a lower risk of cardiovascular disease and diabetes. This in vitro study determined the effect of individual fatty acids (FA) found in dairy, and FA mixtures representative of a high SFA and a low SFA dairy lipid on markers of endothelial function in healthy and type II diabetic aortic endothelial cells. METHODS: Cells were incubated for 24 h with FA mixtures (400 μM) and individual FA: oleic acid (OA; 150 μM); palmitic acid (PA; 150 μM); stearic acid (SA: 40 μM); trans-palmitelaidic acid (trans-PA; 20 μM); trans-vaccenic acid (trans-VA; 20 μM); α-linolenic acid (ALA; 20 μM) and linoleic acid (LA; 20 μM). Cellular adhesion molecules (sICAM-1, sVCAM-1 and sE-selectin) and nitric oxide (NO) were measured using ELISA and a chemiluminescent-based assay, respectively. Relative gene expression of these markers, including the insulin receptor, was performed using real-time PCR as well as FA compositions of cell pellets by gas chromatography. RESULTS: FA mixtures affected sE-selectin concentrations (P = 0.008), with concentrations lower following the high SFA compared to the low SFA mixture (P = 0.004), while NO concentrations were higher in diabetic compared to healthy cells (P = 0.029). Individual FA affected NO (P = 0.007) and sE-selectin (P = 0.040) concentrations with an increase following PA incubation relative to all other FA treatments (P < 0.05). PA increased sE-selectin compared with other FA treatments (P < 0.05). sE-selectin concentrations were also higher in healthy compared to diabetic cells (P = 0.023). Expression of ICAM-1 and insulin receptor was up-regulated in healthy compared to diabetic cells (P = 0.014 and P = 0.006 respectively). CONCLUSIONS: Healthy and type II diabetic cells respond differently to incubation with FA treatments. Overall, physiological concentrations of dairy FA, but not dairy FA mixtures, substantially affected markers of endothelial function.
Language eng
DOI 10.1016/j.atherosclerosis.2014.02.015
Field of Research 111199 Nutrition and Dietetics not elsewhere classified
1103 Clinical Sciences
1102 Cardiovascular Medicine And Haematology
Socio Economic Objective 920411 Nutrition
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Elsevier Ireland
Persistent URL http://hdl.handle.net/10536/DRO/DU:30082999

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