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Elemental and metabolite profiling of nickel hyperaccumulators from New Caledonia

Callahan, Damien L., Roessner, Ute, Dumontet, Vincent, De Livera, Alysha M., Doronila, Augustine, Baker, Alan J.M. and Kolev, Spas D. 2012, Elemental and metabolite profiling of nickel hyperaccumulators from New Caledonia, Phytochemistry, vol. 81, pp. 80-89, doi: 10.1016/j.phytochem.2012.06.010.

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Title Elemental and metabolite profiling of nickel hyperaccumulators from New Caledonia
Author(s) Callahan, Damien L.
Roessner, Ute
Dumontet, Vincent
De Livera, Alysha M.
Doronila, Augustine
Baker, Alan J.M.
Kolev, Spas D.
Journal name Phytochemistry
Volume number 81
Start page 80
End page 89
Total pages 10
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2012-09
ISSN 0031-9422
Keyword(s) Nickel hyperaccumulation
Metabolomics
GC–MS
ICP
New Caledonia
Serpentine
Summary Leaf material from nine Ni hyperaccumulating species was collected in New Caledonia: Homalium kanaliense (Vieill.) Briq., Casearia silvana Schltr, Geissois hirsuta Brongn. & Gris, Hybanthus austrocaledonicusSeem, Psychotria douarrei (G. Beauvis.) Däniker, Pycnandra acuminata (Pierre ex Baill.) Swenson & Munzinger (syn Sebertia acuminata Pierre ex Baill.), Geissois pruinosa Brongn. & Gris, Homalium deplanchei (Viell) Warb. and Geissois bradfordii (H.C. Hopkins). The elemental concentration was determined by inductively-coupled plasma optical emission spectrometry (ICP-OES) and from these results it was foundthat the species contained Ni concentrations from to 250–28,000 mg/kg dry mass. Gas chromatography mass spectrometry (GC–MS)-based metabolite profiling was then used to analyse leaves of each species.The aim of this study was to target Ni-binding ligands through correlation analysis of the metabolite levels and leaf Ni concentration. Approximately 258 compounds were detected in each sample. As has been observed before, a correlation was found between the citric acid and Ni concentrations in the leaves for all species collected. However, the strongest Ni accumulator, P. douarrei, has been found to contain particularly high concentrations of malonic acid, suggesting an additional storage mechanism for Ni. A size exclusion chromatography separation protocol for the separation of Ni-complexes in P. acuminata sap was also applied to aqueous leaf extracts of each species. A number of metabolites were identified in complexes with Ni including Ni-malonate from P. douarrei. Furthermore, the levels for some metabolites were found to correlate with the leaf Ni concentration. These data show that Ni ions can be bound by a range of small molecules in Ni hyperaccumulation in plants.
Language eng
DOI 10.1016/j.phytochem.2012.06.010
Field of Research 060705 Plant Physiology
060101 Analytical Biochemistry
030201 Bioinorganic Chemistry
03 Chemical Sciences
06 Biological Sciences
Socio Economic Objective 960809 Mining Flora
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2012, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30083094

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