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Comprehensive profiling and quantitation of amine group containing metabolites

Boughton, Berin A., Callahan, Damien L., Silva, Claudio, Bowne, Jairus, Nahid, Amsha, Rupasinghe, Thusita, Tull, Dedreja L., McConville, Malcolm J., Bacic, Antony and Roessner, Ute 2011, Comprehensive profiling and quantitation of amine group containing metabolites, Analytical chemistry, vol. 83, no. 19, pp. 7523-7530, doi: 10.1021/ac201610x.

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Title Comprehensive profiling and quantitation of amine group containing metabolites
Author(s) Boughton, Berin A.
Callahan, Damien L.
Silva, Claudio
Bowne, Jairus
Nahid, Amsha
Rupasinghe, Thusita
Tull, Dedreja L.
McConville, Malcolm J.
Bacic, Antony
Roessner, Ute
Journal name Analytical chemistry
Volume number 83
Issue number 19
Start page 7523
End page 7530
Total pages 8
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2011
ISSN 0003-2700
1520-6882
Summary Primary and secondary amines, including amino acids, biogenic amines, hormones, neurotransmitters, and plant siderophores, are readily derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate using easily performed experimental methodology. Complex mixtures of these amine derivatives can be fractionated and quantified using liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI-MS). Upon collision induced dissociation (CID) in a quadrupole collision cell, all derivatized compounds lose the aminoquinoline tag. With the use of untargeted fragmentation scan functions, such as precursor ion scanning, the loss of the aminoquinoline tag (Amq) can be monitored to identify derivatized species; and the use of targeted fragmentation scans, such as multiple reaction monitoring, can be exploited to quantitate amine-containing molecules. Further, with the use of accurate mass, charge state, and retention time, identification of unknown amines is facilitated. The stability of derivatized amines was found to be variable with oxidatively labile derivatives rapidly degrading. With the inclusion of antioxidant and reducing agents, tris(2-carboxyethyl)-phosphine (TCEP) and ascorbic acid, into both extraction solvents and reaction buffers, degradation was significantly decreased, allowing reproducible identification and quantification of amine compounds in large sample sets.
Language eng
DOI 10.1021/ac201610x
Field of Research 030101 Analytical Spectrometry
030108 Separation Science
060101 Analytical Biochemistry
0301 Analytical Chemistry
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2011, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30083156

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