You are not logged in.
Openly accessible

Constitutive overexpression of the OsNAS gene family reveals single-gene strategies for effective iron- and zinc-biofortification of rice endosperm

Johnson, Alexander A.T., Kyriacou, Bianca, Callahan, Damien L., Carruthers, Lorraine, Stangoulis, James, Lombi, Enzo and Tester, Mark 2011, Constitutive overexpression of the OsNAS gene family reveals single-gene strategies for effective iron- and zinc-biofortification of rice endosperm, PLoS one, vol. 6, no. 9, Article Number : e24476, pp. 1-11, doi: 10.1371/journal.pone.0024476.

Attached Files
Name Description MIMEType Size Downloads
callahan-constitutive-2011.pdf Published version application/pdf 2.83MB 25

Title Constitutive overexpression of the OsNAS gene family reveals single-gene strategies for effective iron- and zinc-biofortification of rice endosperm
Formatted title Constitutive overexpression of the OsNAS gene family reveals single-gene strategies for effective iron- and zinc-biofortification of rice endosperm
Author(s) Johnson, Alexander A.T.
Kyriacou, Bianca
Callahan, Damien L.ORCID iD for Callahan, Damien L. orcid.org/0000-0002-6384-8717
Carruthers, Lorraine
Stangoulis, James
Lombi, Enzo
Tester, Mark
Contributor(s) Baxter, Ivan
Journal name PLoS one
Volume number 6
Issue number 9
Season Article Number : e24476
Start page 1
End page 11
Total pages 11
Publisher Public Library of Science (PLoS)
Place of publication San Francisco, Calif.
Publication date 2011
ISSN 1932-6203
Summary Background
Rice is the primary source of food for billions of people in developing countries, yet the commonly consumed polished grain contains insufficient levels of the key micronutrients iron (Fe), zinc (Zn) and Vitamin A to meet daily dietary requirements. Experts estimate that a rice-based diet should contain 14.5 µg g−1 Fe in endosperm, the main constituent of polished grain, but breeding programs have failed to achieve even half of that value. Transgenic efforts to increase the Fe concentration of rice endosperm include expression of ferritin genes, nicotianamine synthase genes (NAS) or ferritin in conjunction with NAS genes, with results ranging from two-fold increases via single-gene approaches to six-fold increases via multi-gene approaches, yet no approach has reported 14.5 µg g−1 Fe in endosperm.

Methodology/Principal Findings
Three populations of rice were generated to constitutively overexpress OsNAS1, OsNAS2 or OsNAS3, respectively. Nicotianamine, Fe and Zn concentrations were significantly increased in unpolished grain of all three of the overexpression populations, relative to controls, with the highest concentrations in the OsNAS2 and OsNAS3 overexpression populations. Selected lines from each population had at least 10 µg g−1 Fe in polished grain and two OsNAS2 overexpression lines had 14 and 19 µg g−1 Fe in polished grain, representing up to four-fold increases in Fe concentration. Two-fold increases of Zn concentration were also observed in the OsNAS2 population. Synchrotron X-ray fluorescence spectroscopy demonstrated that OsNAS2 overexpression leads to significant enrichment of Fe and Zn in phosphorus-free regions of rice endosperm.

Conclusions
The OsNAS genes, particularly OsNAS2, show enormous potential for Fe and Zn biofortification of rice endosperm. The results demonstrate that rice cultivars overexpressing single rice OsNAS genes could provide a sustainable and genetically simple solution to Fe and Zn deficiency disorders affecting billions of people throughout the world.
Language eng
DOI 10.1371/journal.pone.0024476
Field of Research 060702 Plant Cell and Molecular Biology
Socio Economic Objective 820402 Rice
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2011, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30083158

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 91 times in TR Web of Science
Scopus Citation Count Cited 116 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 93 Abstract Views, 27 File Downloads  -  Detailed Statistics
Created: Thu, 05 May 2016, 14:33:09 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.