Metabolite profiling reveals distinct changes in carbon and nitrogen metabolism in phosphate-deficient barley plants (Hordeum vulgare L.)

Huang, Chun Y., Roessner, Ute, Eickmeier, Ira, Genc, Yusuf, Callahan, Damien L., Shirley, Neil, Langridge, Peter and Bacic, Antony 2008, Metabolite profiling reveals distinct changes in carbon and nitrogen metabolism in phosphate-deficient barley plants (Hordeum vulgare L.), Plant and cell physiology, vol. 49, no. 5, pp. 691-703, doi: 10.1093/pcp/pcn044.

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Title Metabolite profiling reveals distinct changes in carbon and nitrogen metabolism in phosphate-deficient barley plants (Hordeum vulgare L.)
Author(s) Huang, Chun Y.
Roessner, Ute
Eickmeier, Ira
Genc, Yusuf
Callahan, Damien L.ORCID iD for Callahan, Damien L.
Shirley, Neil
Langridge, Peter
Bacic, Antony
Journal name Plant and cell physiology
Volume number 49
Issue number 5
Start page 691
End page 703
Total pages 13
Publisher Oxford Journals
Place of publication Oxford, Eng.
Publication date 2008
ISSN 0032-0781
Keyword(s) Ammonium
Barley (Hordeum vulgare L.)
Metabolite profile
Phosphate deficiency
Summary Plants modify metabolic processes for adaptation to low phosphate (P) conditions. Whilst transcriptomic analyses show that P deficiency changes hundreds of genes related to various metabolic processes, there is limited information available for global metabolite changes of P-deficient plants, especially for cereals. As changes in metabolites are the ultimate ‘readout’ of changes in gene expression, we profiled polar metabolites from both shoots and roots of P-deficient barley (Hordeum vulgare) using gas chromatography–mass spectrometry (GC-MS). The results showed that mildly P-deficient plants accumulated di- and trisaccharides (sucrose, maltose, raffinose and 6-kestose), especially in shoots. Severe P deficiency increased the levels of metabolites related to ammonium metabolism in addition to di- and trisaccharides, but reduced the levels of phosphorylated intermediates (glucose-6-P, fructose-6-P, inositol-1-P and glycerol-3-P) and organic acids (α-ketoglutarate, succinate, fumarate and malate). The results revealed that P-deficient plants modify carbohydrate metabolism initially to reduce P consumption, and salvage P from small P-containing metabolites when P deficiency is severe, which consequently reduced levels of organic acids in the tricarboxylic acid (TCA) cycle. The extent of the effect of severe P deficiency on ammonium metabolism was also revealed by liquid chromatography–mass spectrometry (LC-MS) quantitative analysis of free amino acids. A sharp increase in the concentrations of glutamine and asparagine was observed in both shoots and roots of severely P-deficient plants. Based on these data, a strategy for improving the ability of cereals to adapt to low P environments is proposed that involves alteration in partitioning of carbohydrates into organic acids and amino acids to enable more efficient utilization of carbon in P-deficient plants.
Language eng
DOI 10.1093/pcp/pcn044
Field of Research 060705 Plant Physiology
0607 Plant Biology
0601 Biochemistry And Cell Biology
Socio Economic Objective 820501 Barley
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2008, Oxford Journals
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