Bio-conjugation of antioxidant peptide on surface-modified gold nanoparticles: a novel approach to enhance the radical scavenging property in cancer cell

Kalmodia, Sushma, Vandhana, Suryanarayanan, Tejaswini Rama, B.R., Jayashree, Balasubramanyam, Sreenivasan Seethalakshmi, T., Umashankar, Vetrivel, Yang, Wenrong, Barrow, Colin J., Krishnakumar, Subramanian and Elchuri, Sailaja V. 2016, Bio-conjugation of antioxidant peptide on surface-modified gold nanoparticles: a novel approach to enhance the radical scavenging property in cancer cell, Cancer nanotechnology, vol. 7, no. 1, pp. 1-19, doi: 10.1186/s12645-016-0013-x.

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Title Bio-conjugation of antioxidant peptide on surface-modified gold nanoparticles: a novel approach to enhance the radical scavenging property in cancer cell
Author(s) Kalmodia, Sushma
Vandhana, Suryanarayanan
Tejaswini Rama, B.R.
Jayashree, Balasubramanyam
Sreenivasan Seethalakshmi, T.
Umashankar, Vetrivel
Yang, WenrongORCID iD for Yang, Wenrong orcid.org/0000-0001-8815-1951
Barrow, Colin J.ORCID iD for Barrow, Colin J. orcid.org/0000-0002-2153-7267
Krishnakumar, Subramanian
Elchuri, Sailaja V.
Journal name Cancer nanotechnology
Volume number 7
Issue number 1
Start page 1
End page 19
Total pages 19
Publisher Springer
Place of publication Berlin, Germany
Publication date 2016-02-09
ISSN 1868-6966
Keyword(s) antioxidant peptide
conjugate
cancer cell
gold nanoparticles (GNPs)
oxidative stress
reactive oxygen species (ROS)
Summary BACKGROUND: Functionalized gold nanoparticles are emerging as a promising nanocarrier for target specific delivery of the therapeutic molecules in a cancer cell, as a result it targeted selectively to the cancer cell and minimized the off-target effect. The functionalized nanomaterial (bio conjugate) brings novel functional properties, for example, the high payload of anticancer, antioxidant molecules and selective targeting of the cancer molecular markers. The current study reported the synthesis of multifunctional bioconjugate (GNPs-Pep-A) to target the cancer cell. METHODS: The GNPs-Pep-A conjugate was prepared by functionalization of GNPs with peptide-A (Pro-His-Cys-Lys-Arg-Met; Pep-A) using thioctic acid as a linker molecule. The GNPs-Pep-A was characterized and functional efficacy was tested using Retinoblastoma (RB) cancer model in vitro. RESULTS: The GNPs-Pep-A target the reactive oxygen species (ROS) in RB, Y79, cancer cell more effectively, and bring down the ROS up to 70 % relative to control (untreated cells) in vitro. On the other hand, Pep-A and GNPs showed 40 and 9 % reductions in ROS, respectively, compared to control. The effectiveness of bioconjugate indicates the synergistic effect, due to the coexistence of both organic (Pep-A) and inorganic phase (GNPs) in novel GNPs-Pep-A functional material. In addition to this, it modulates the mRNA expression of antioxidant genes glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT) by two-threefolds as observed. CONCLUSIONS: The effects of GNPs-Pep-A on ROS reduction and regulation of antioxidant genes confirmed that Vitis vinifera L. polyphenol-coated GNPs synergistically improve the radical scavenging properties and enhanced the apoptosis of cancer cell.
Language eng
DOI 10.1186/s12645-016-0013-x
Field of Research 030302 Nanochemistry and Supramolecular Chemistry
060103 Cell Development, Proliferation and Death
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30083310

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