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Exploring the effects of omega-3 and omega-6 fatty acids on allergy using a HEK-blue cell line.

Ahmed, Nayyar, Barrow, Colin and Suphioglu, Cenk 2016, Exploring the effects of omega-3 and omega-6 fatty acids on allergy using a HEK-blue cell line., International journal of molecular science, vol. 17, no. 2, pp. 220-231, doi: 10.3390/ijms17020220.

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Title Exploring the effects of omega-3 and omega-6 fatty acids on allergy using a HEK-blue cell line.
Author(s) Ahmed, Nayyar
Barrow, ColinORCID iD for Barrow, Colin orcid.org/0000-0002-2153-7267
Suphioglu, CenkORCID iD for Suphioglu, Cenk orcid.org/0000-0003-0101-0668
Journal name International journal of molecular science
Volume number 17
Issue number 2
Start page 220
End page 231
Total pages 11
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2016-02-06
ISSN 1422-0067
Keyword(s) fatty acids
atopy
cytokines
IL-4 receptor
IL-13 receptor
STAT6 pathway
IgE antibody
SEAP
HEK-Blue
Summary BACKGROUND: Allergic reactions can result in life-threatening situations resulting in high economic costs and morbidity. Therefore, more effective reagents are needed for allergy treatment. A causal relationship has been suggested to exist between the intake of omega-3/6 fatty acids, such as docosahexanoic acid (DHA), eicosapentanoic acid (EPA), docosapentanoic acid (DPA) and arachidonic acid (AA), and atopic individuals suffering from allergies. In allergic cascades, the hallmark cytokine IL-4 bind to IL-4 receptor (IL-4R) and IL-13 binds to IL-13 receptor (IL-13R), this activates the STAT6 phosphorylation pathway leading to gene activation of allergen-specific IgE antibody production by B cells. The overall aim of this study was to characterize omega-3/6 fatty acids and their effects on STAT6 signaling pathway that results in IgE production in allergic individuals.

METHODS: The fatty acids were tested in vitro with a HEK-Blue IL-4/IL-13 reporter cell line model, transfected with a reporter gene that produces an enzyme, secreted embryonic alkaline phosphatase (SEAP). SEAP acts as a substitute to IgE when cells are stimulated with bioactive cytokines IL-4 and/or IL-13.

RESULTS: We have successfully used DHA, EPA and DPA in our studies that demonstrated a decrease in SEAP secretion, as opposed to an increase in SEAP secretion with AA treatment. A statistical Student's t-test revealed the significance of the results, confirming our initial hypothesis.

CONCLUSION: We have successfully identified and characterised DHA, EPA, DPA and AA in our allergy model. While AA was a potent stimulator, DHA, EPA and DPA were potential inhibitors of IL-4R/IL-13R signalling, which regulates the STAT6 induced pathway in allergic cascades. Such findings are significant in the future design of dietary therapeutics for the treatment of allergies.
Language eng
DOI 10.3390/ijms17020220
Field of Research 110701 Allergy
060101 Analytical Biochemistry
0399 Other Chemical Sciences
060406 Genetic Immunology
069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30083311

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.