Elite hairy roots of Ocimum basilicum as a new source of rosmarinic acid and antioxidants

Srivastava, Shivani, Conlan, Xavier, Adholeya, Alok and Cahill, David 2016, Elite hairy roots of Ocimum basilicum as a new source of rosmarinic acid and antioxidants, Plant cell, tissue and organ culture, vol. 126, no. 1, pp. 19-32, doi: 10.1007/s11240-016-0973-x.

Attached Files
Name Description MIMEType Size Downloads

Title Elite hairy roots of Ocimum basilicum as a new source of rosmarinic acid and antioxidants
Author(s) Srivastava, Shivani
Conlan, XavierORCID iD for Conlan, Xavier orcid.org/0000-0003-0829-0551
Adholeya, Alok
Cahill, DavidORCID iD for Cahill, David orcid.org/0000-0002-2556-0528
Journal name Plant cell, tissue and organ culture
Volume number 126
Issue number 1
Start page 19
End page 32
Total pages 14
Publisher Springer
Place of publication Berlin, Germany
Publication date 2016-07
ISSN 1573-5044
1573-5044
Keyword(s) Antioxidants
Chemiluminescence
Hairy roots
Ocimum basilicum
Morphotyping
Rosmarinic acid
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Plant Sciences
POTASSIUM-PERMANGANATE CHEMILUMINESCENCE
AGROBACTERIUM-RHIZOGENES A4
TRANSFORMED ROOTS
SECONDARY METABOLISM
METHYL JASMONATE
ROL GENES
CULTURES
INDUCTION
PHENOTYPES
GROWTH
Summary This study reports Agrobacterium rhizogenes-mediated transformation of three cultivars of Ocimum basilicum for hairy root establishment, screening and selection for the production of rosmarinic acid and antioxidants. Hairy root development was found to be explant-specific and virulence-dependent. Distinct inter-cultivar morphological variability was found between the seven axenically developed hairy root lines and morphological traits were found to be correlated with the presence of aux2 genes, their expression and endogenous IAA content. Further inter-cultivar variability in the content of total phenolics, rosmarinic acid and caffeic acid was also found. Production of rosmarinic acid was found to be age-dependent and cultivar-specific. Chemiluminescence analysis showed the hairy roots to be rich in antioxidants and that rosmarinic acid was the major antioxidant molecule. The concentration of rosmarinic acid was found to be positively correlated with the total antioxidant potential of the hairy root extracts. On the basis of origin, morphology and metabolite content, three elite hairy root lines were selected that had significantly higher rosmarinic acid production, biomass and antioxidant potential than non-transformed roots. These new lines are rich reserves of both antioxidants and rosmarinic acid.
Language eng
DOI 10.1007/s11240-016-0973-x
Field of Research 060702 Plant Cell and Molecular Biology
0607 Plant Biology
0703 Crop And Pasture Production
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Springer Science+Business Media
Persistent URL http://hdl.handle.net/10536/DRO/DU:30083359

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in TR Web of Science
Scopus Citation Count Cited 8 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 295 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Thu, 14 Jul 2016, 21:36:27 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.