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Understanding the influence of key ionic liquid properties on the hydrolytic activity of thermomyces lanuginosus lipase

Han, Qi, Wang, Xungai and Byrne, Nolene 2016, Understanding the influence of key ionic liquid properties on the hydrolytic activity of thermomyces lanuginosus lipase, Chemcatchem, vol. 8, no. 8, pp. 1551-1556, doi: 10.1002/cctc.201600014.

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Title Understanding the influence of key ionic liquid properties on the hydrolytic activity of thermomyces lanuginosus lipase
Author(s) Han, Qi
Wang, Xungai
Byrne, Nolene
Journal name Chemcatchem
Volume number 8
Issue number 8
Start page 1551
End page 1556
Total pages 6
Publisher Wiley-Blackwell
Place of publication Chichester, Eng.
Publication date 2016-04-20
ISSN 1867-3880
1867-3899
Keyword(s) Science & Technology
Physical Sciences
Chemistry, Physical
Chemistry
enzyme catalysis
hydrolases
ionic liquids
solvent effects
water
Summary Ionic liquids (ILs) are solvents with numerous properties, which have been recently used for enzyme catalysis. In this work, five different ILs based on primary, tertiary, and quaternary ammonium cations coupled with mesylate and propionate anions were used as media for hydrolysis by the industrially relevant enzyme Thermomyces lanuginosus lipase (TLL). We correlated the TLL activity with various key IL and IL-water properties, including ion concentration, water activity (aw), kosmotropicity, hydrogen-bond basicity (β), and pH. The ion concentration was associated with aw, and the molar ratio of water/IL 5:1 (aw≈0.6) was found to be the threshold for assured TLL activity. Triethylammonium mesylate was the best IL owing to its kosmotropicity and ideal intrinsic β. The pH of IL-water mixtures is a key parameter related to the conformational change of TLL. We demonstrated the pH effect of the IL-water mixtures can be overcome by buffering, and the buffered system displayed the greatest activity.
Language eng
DOI 10.1002/cctc.201600014
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Wiley-VCH Verlag GmbH & Co. KGaA Weinheim
Persistent URL http://hdl.handle.net/10536/DRO/DU:30083672

Document type: Journal Article
Collection: Institute for Frontier Materials
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