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Eicosapentaenoic acid, arachidonic acid and eicosanoid metabolism in juvenile barramundi Lates calcarifer

Salini, Michael, Wade, Nicholas M., Araújo, Bruno C., Turchini, Giovanni M. and Glencross, Brett D. 2016, Eicosapentaenoic acid, arachidonic acid and eicosanoid metabolism in juvenile barramundi Lates calcarifer, Lipids, vol. 51, no. 8, pp. 973-988, doi: 10.1007/s11745-016-4167-4.

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Title Eicosapentaenoic acid, arachidonic acid and eicosanoid metabolism in juvenile barramundi Lates calcarifer
Author(s) Salini, Michael
Wade, Nicholas M.
Araújo, Bruno C.
Turchini, Giovanni M.ORCID iD for Turchini, Giovanni M. orcid.org/0000-0003-0694-4283
Glencross, Brett D.
Journal name Lipids
Volume number 51
Issue number 8
Start page 973
End page 988
Total pages 16
Publisher Springer
Place of publication Berlin, Germany
Publication date 2016-08
ISSN 1558-9307
1558-9307
Keyword(s) Arachidonic acid
Cyclooxygenase
Eicosanoids
Gene expression
Lipoxygenase
Molecular cloning
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Nutrition & Dietetics
TURBOT SCOPHTHALMUS-MAXIMUS
FUNCTIONAL CYCLOOXYGENASE-2 GENES
POLYUNSATURATED FATTY-ACIDS
SOLEA-SENEGALENSIS LARVAE
MARINE FISH
DOCOSAHEXAENOIC ACIDS
LIPID-COMPOSITION
ATLANTIC SALMON
STRESS-RESPONSE
PAGRUS-AURATUS
Summary A two part experiment was conducted to assess the response of barramundi (Lates calcarifer; initial weight = 10.3 ± 0.03 g; mean ± S.D.) fed one of five diets with varying eicosapentaenoic acid (diets 1, 5, 10, 15 and 20 g/kg) or one of four diets with varying arachidonic acid (1, 6, 12, 18 g/kg) against a fish oil control diet. After 6 weeks of feeding, the addition of EPA or ARA did not impact on growth performance or feed utilisation. Analysis of the whole body fatty acids showed that these reflected those of the diets. The ARA retention demonstrated an inversely related curvilinear response to either EPA or ARA. The calculated marginal utilisation efficiencies of EPA and ARA were high (62.1 and 91.9 % respectively) and a dietary ARA requirement was defined (0.012 g/kg(0.796)/day). The partial cDNA sequences of genes regulating eicosanoid biosynthesis were identified in barramundi tissues, namely cyclooxygenase 1 (Lc COX1a, Lc COX1b), cyclooxygenase 2 (Lc COX2) and lipoxygenase (Lc ALOX-5). Both Lc COX2 and Lc ALOX-5 expression in the liver tissue were elevated in response to increasing dietary ARA, meanwhile expression levels of Lc COX2 and the mitochondrial fatty acid oxidation gene carnitine palmitoyltransferase 1 (Lc CPT1a) were elevated in the kidney. A low level of EPA increased the expression of Lc COX1b in the liver. Consideration should be given to the EPA to ARA balance for juvenile barramundi in light of nutritionally inducible nature of the cyclooxygenase and lipoxygenase enzymes.
Language eng
DOI 10.1007/s11745-016-4167-4
Field of Research 070401 Aquaculture
Socio Economic Objective 830102 Aquaculture Fin Fish (excl. Tuna)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, AOCS
Persistent URL http://hdl.handle.net/10536/DRO/DU:30084279

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